V.S. Sibirtsev1, K.I. Evgenova2, E.K. Sinichenko3, A.Yu. Zaitseva4, S.N. Glebov5, V.V. Grishin6, S.N. Barmashov7

1–3 Saint-Petersburg State Chemical and Pharmaceutical University (Saint Petersburg, Russia)

1,4,5 Institute for Analytical Instrumentation of the RAS (Saint Petersburg, Russia)

6 National state university of physical education, sports and health (Saint Petersburg, Russia)

7 First Saint Petersburg state medical university (Saint Petersburg, Russia)

1 vs1969r@mail.ru, 2 kseniya.evgenova@spcpu.ru, 3 ekaterina.sinichenko@spcpu.ru, 4 anna@da-24.ru, 5 stepangleboff@yandex.ru, 6 vladimir.grishin@pharminnotech.com, 7 barmashovs2309@gmail.com

The problem of quality control of various pharmaceutical, food and other products (including those containing various plant extracts) has recently become increasingly relevant.

Purpose of this study – development and testing of a new rapid and widely available method for instrumental assessment of the pre- and antimicrobial properties of samples of various pharmaceutical, food and other products, as well as ingredients and additives to them.

The methodology presented in this work consists of periodic (in this case, after 0, 40, 80 and 120 minutes) recording of changes in pH, as well as potential differences between the silver chloride reference electrode and the indicator iron and brass electrodes of the “first kind” (for which we have developed two fundamentally new potentiometric sensors), characteristic of a liquid nutrient medium incubated in the presence and in the absence of test samples (TS) and viable test microorganisms (TM), for which in this work we used Escherichia coli, which are typical representatives of human and natural microflora.

Using this method, we conducted a comparative analysis of the biological activity of 9 different industrially produced carbon dioxide plant extracts depending on both the type of plant material from which these extracts were obtained and the concentration of these extracts in the test medium, as well as the time of their interaction with the TM (for which we introduced a new additional parameter that takes into account the dynamics of changes in the biological activity of the TS during their interaction with the TM).

The conducted study confirmed the higher speed, information content and cost-effectiveness of the method presented in this work in comparison with both classical “visual” and previously used instrumental microbiological methods, applied both in quality control of existing and in the development of new pharmaceutical, food and other products, as well as ingredients and additives to them.

Sibirtsev V.S., Evgenova K.I., Sinichenko E.K., Zaitseva A.Yu., Glebov S.N., Grishin V.V., Barmashov S.N. Methodology of potentiometric microbiological testing as applied to the comparative analysis of the carbon dioxide plant extracts. Technologies of Living Systems. 2026. V. 23. № 3. Р. 78-89. DOI: https://doi.org/10.18127/j20700997-202603-08 (In Russian).

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