V.A. Mangusheva1, D.V. Ivaschenko2, M.A. Vigovsky3, N.I. Kalinina4, L.N. Shcherbakova5, P.I. Makarevich6, V.Yu. Sysoeva7

1–7 Medical Scientific and Educational Institute, Lomonosov Moscow State University (Moscow, Russia)

1 veronikamanguseva@gmail.com, 2 nanomy_1@icloud.com, 3 vigovskiy_m.a@mail.ru, 4 n_i_kalinina@mail.ru, 5 liya.fbm@gmail.com, 6 pavel.makarevich@gmail.com, 7 veroniks@mail.ru

Endometrial mesenchymal stromal cells (EMSCs) play a key role in regulating the cyclical turnover of the uterine lining. These cells can control endometrial proliferation, so an imbalance in their activity may lead to pathological processes rather than physiological endometrial restoration. Studying the regulation of MSCs' functional activity requires the development of a reliable in vitro model.

Aim of the study – to develop a method for isolating and culturing MSCs from endometrial biopsies and to compare the differentiation potential of endometrial stromal cells from fertile women and patients with repeated implantation failures of genetically healthy embryos.

MSCs were isolated from endometrial biopsies of a high-parity patient using a modified technique. The differentiation capacity of MSCs was assessed by culturing the cells in induction media and subsequent histochemical staining. The isolation method used yielded a sufficient number of viable cells that rapidly adhered to the plastic. MSCEMs were capable of osteogenic and chondrogenic differentiation, but their ability to accumulate lipid droplets was insignificant. A comparative analysis of MSCEM differentiation in fertile women and patients with repeated implantation failures of genetically healthy embryos revealed reduced osteogenic differentiation efficiency in the latter.

An in vitro model for studying the regulation of MSCEM functional activity is proposed. Data obtained using this model may be key to the development of new diagnostic and treatment methods for pathologies associated with endometrial dysfunction, including infertility and hyperplasia. Use of this model will expand fundamental knowledge about the contribution of these cells to endometrial renewal and growth.

Mangusheva V.A., Ivaschenko D.V., Vigovsky M.A., Kalinina N.I., Shcherbakova L.N., Makarevich P.I., Sysoeva V.Yu. Differentiation Features of Cultured Endometrial Mesenchymal Stromal Cells. Technologies of Living Systems. 2026. V. 23. № 2. Р. 91-99. DOI: https://doi.org/10.18127/j20700997-202602-09 (In Russian).

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