A.V. Lokarev1, V.M. Popova2, I.N. Matveeva3, S.A. Grin4, A.D. Zaberezhny5, E.V. Markova6, S.A. Vasiliev7

1–7 All-Russian Research and Technological Institute of Biological Industry (Biokombinata village, Losino-Petrovsky urban district, Moscow Region, Russia)

1 eko-plus@mail.ru, 2 burevestnik46@mail.ru, 3 biolog1967@mail.ru, 4 grinnsvetlana@mail.ru, 5 zaberezhny@mail.ru, 6 biologm2020@mail.ru, 7 stepanvasilev0451@gmail.com

An urgent problem for the prevention and treatment of many diseases of animals and humans is the development of original medicines using biotechnological methods. Liposomes in combination with a new generation of drugs created due to the success of protein and genetic engineering and plant extracts are promising. The use of liposome drugs in medicine and veterinary medicine is the subject of close attention.

The development of medicines requires the solution of specific technological issues: for example, to create phyto ointment for the treatment of arthritis, arthrosis, fungal infections, or ointment with collagenase, it is necessary to select active components with wound healing, antimicrobial, anti-inflammatory properties, antifungal properties, as well as a complex of optimal excipients that provide the necessary biopharmaceutical characteristics of these compositions. We proposed the composition of a complex extract which contained an alcohol-water extract from 13 plant components in a certain ratio. To enhance anti-arthritic and anti-arthrosis activity, chondroitin sulfate, glucosamine hydrochloride, shilajit, sea buckthorn oil and oil extract from therapeutic mud were additionally included in the developed ointments. Various methods of liposome preparation with the maximum inclusion of complex alcohol-water extract from medicinal plant raw materials were studied. The criteria for comparing the drugs included in the internal volume of the vesicle were: the percentage of the component, the size of liposomes, the simplicity and accessibility of the method.

Lokarev A.V., Popova V.M., Matveeva I.N., Grin S.A., Zaberezhny A.D., Markova E.V., Vasiliev S.A. Development of medicines from plant raw materials and biologically active natural components. Technologies of Living Systems. 2025. V. 22. № 4. Р. 107-120. DOI: https://doi.org/10.18127/j20700997-202504-12 (In Russian).

1. Bombardier C., Laine L., Reicin A. et al. Comparison of upper gastrointestinal toxicity of rofecoxib and naproxen in patients with rheumatoid arthritis. New England Journal of Medicine. 2000. V. 343. № 21. P. 1520–1528.
2. Lokarev A.V., Ogaj M.A., Slivkin A.I., Belenova A.S. Razrabotka i reologicheskie issledovaniya mazej s kompleksnym ekstraktom iz LRS. Vestnik Voronezhskogo gosudarstvennogo universiteta. Ser. Himiya, biologiya, farmaciya. 2019. № 2. S. 97–101. (in Russian).
3. Patent № 2007114164 (RF) Sposob polucheniya liposom. Zayavka № 2007114164/15; zayavleno 17.04.2007; opubl. 27.05.2008, Byul. № 15. 5 s. (in Russian).
4. Batyrkhanov Sh.K., Imambaeva T.M., Karimkhanova A.T., Abdullaeva G.M. Role of phytotherapy in modern medicine. Medicine of Kyrgyzstan. 2015. V. 1. № 4. P. 30–32.
5. Lokarev A., Matveeva I., Markova E. et al. Biomathematical approach determination of a rational formula of complex phytocompositions. BIO Web of Conferences. EBWFF 2024 – International Scientific Conference Ecological and Biological Well-Being of Flora and Fauna. Les Ulis. 2024. P. 01004.
6. Malovastyj K.S., Torikov V.E., Meshkov I.I. Fitoterapiya v veterinarii, tradicionnoj i netradicionnoj medicine. M.: Feniks. 2007. 84 s. (in Russian).
7. Poturaj V.A. Organicheskoe veshchestvo v termal'nyh i poverhnostnyh vodah Tumninskogo mestorozhdeniya termal'nyh vod, Dal'nij Vostok. Izvestiya Tomskogo politekhnicheskogo universiteta. 2014. T. 324. № 3. S. 44–52. (in Russian).
8. Lokarev A., Potapov V., Matveeva I. et al. Mathematical model of thermal water composition. BIO Web of Conferences. EBWFF 2024 – International Scientific Conference Ecological and Biological Well-Being of Flora and Fauna. Les Ulis. 2024. P. 03025.
9. Daily J.W., Yang M., Park S. Efficacy of Turmeric Extracts and Curcumin for Alleviating the Symptoms of Joint Arthritis: A Systematic Review and Meta-Analysis of Randomized Clinical Trials. Journal of Medicinal Food. 2016. V. 19. № 8. P. 717–729. DOI: 10.1089/jmf.2016.3705
10. Basnyat S., Kolasinski S.L. Ayurvedic Medicine for Rheumatoid Arthritis. Current Rheumatology Reports. 2014. V. 16. № 8. P. 435. DOI: 10.1007/s11926-014-0435-6
11. Ramadan G., El-Menshawy O. Protective Effects of Ginger-Turmeric Rhizomes Mixture on Joint Inflammation, Atherogenesis, Kidney Dysfunction and Other Complications in a Rat Model of Human Rheumatoid Arthritis. International Journal of Rheumatic Diseases. 2013. V. 16. № 2. P. 219–229. DOI:10.1111/1756-185X.12054
12. Lin B., Zhang H., Zhao X.X. et al. Inhibitory Effects of the Root Extract of Litsea cubeba (Lour.) Pers. on Adjuvant Arthritis in Rats. Journal of Ethnopharmacology. 2013. V. 147. № 2. P. 327–334. DOI:10.1016/j.jep.2013.03.011
13. Lokarev A.V., Grin' S.A., Matveeva I.N. i dr. Myagkie liposomal'nye lekarstvennye formy na osnove prirodnyh komponentov. Butlerovskie soobshcheniya. 2024. T. 79. № 7. S. 100–106. DOI: 10.37952/ROI-jbc-01/24-79-7-100 (in Russian).
14. Zhang C.F., Zhang S.L., He X. et al. Antioxidant Effects of Genkwa Flos Flavonoids on Freund’s Adjuvant-Induced Rheumatoid Arthritis in Rats. Journal of Ethnopharmacology. 2014. V. 153. № 3. P. 793–800. DOI:10.1016/j.jep.2014.03.046
15. Kurdyukov E.E., Kuznecova A.V., Semenova E.F., Moiseeva I.YA. K voprosu standartizacii po soderzhaniyu flavonoidov list'ev stevii kak perspektivnogo lekarstvennogo rastitel'nogo syr'ya //Himiya rastitel'nogo syr'ya. 2019. № 1. S. 217–224. (in Russian).
16. Patent № 245698 (RF) Sredstvo, obladayushchee protivovospalitel'nym, protivomikrobnym, reparativnym, a takzhe uluchshayushchim mikrocirkulyaciyu v tkanyah dejstviem i sposob ego polucheniya. Zayavka № 2010127448; prioritet ot 05.07.2010. Zaregistrirovano v Gosreestre izobretenij 20.08.2012. (in Russian).
17. Patent № 2458684 (RF) Gel', obladayushchij protivovospalitel'nym, obezbolivayushchim, ranozazhivlyayushchim dejstviem (varianty). Zayavka № 2010128129; prioritet ot 08.07.2010. Zaregistrirovano v Gosreestre izobretenij 20.08.2012. (in Russian).
18. Patent № 2638798 (RF) Gel' protivogribkovogo dejstviya (varianty). Zayavka № 201707248; prioritet ot 06.03.2017. Zaregistrirovano v Gosreestre izobretenij 15.12.2017. (in Russian).
19. Portugal-Cohen M., Soroka Y., Ma’or Z. et al. Protective Effects of a Novel Preparation Consists of Concentrated Dead Sea Water and Natural Plants Extracts Against Skin Photo-Damage. Journal of Cosmetics, Dermatological Sciences and Applications. 2014. V. 4. № 1. P. 7–15.
20. Seite S. Thermal Waters as Cosmeceuticals: La Roche-Posay Thermal Spring Water Example. Clinical, Cosmetic and Investigational Dermatology. 2013. V. 6. P. 23–28.
21. Novoselov V.S., Novoselov A.V. Racional'nye podhody k terapii poverhnostnyh mikozov. Consilium Medicum. Prilozhenie «Dermatologiya». 2007. T. 9. № 1. S. 24–27. (in Russian).
22. Roohollah M., Shafiee A., Javidnia K. et al. Identification of Antifungal Compounds from Ferula persica var. persica. Pharmaceutical Biology. 2005. V. 43. № 4. P. 293–295.
23. Silva M.R.R., Oliveira J.G.A., Fernandes O.F.L. et al. Antifungal Activity of Ocimum gratissimum Towards Dermatophytes. Mycoses. 2005. V. 48. № 3. P. 172–175.
24. Shuhua G., Yuhua L., Ming Z. et al. Antifungal Activity Various Extracts of Bulbs of Allium cepa. Journal of Guangzhou University of Traditional Chinese Medicine. 2005. V. 22. № 5. P. 379–382.
25. Lokarev A.V., Grin' S.A., Matveeva I.N. Primenenie biologicheskogo skrininga na parameciyah pri razrabotke protivoartritnoj mazi na osnove rastitel'nogo syr'ya. Materialy Mezhdunar. nauchno-prakt. konf., posvyashchennoj 100-letiyu so dnya rozhdeniya Ivana Vasil'evicha Zvyagina. Moskva, 2020. S. 301-305. DOI: 10.47804/978-5-89904-028-3 (in Russian).