A.Yu. Kochergin1, D.S. Kuzichkin2, A.A. Markin3, O.A. Zhuravleva4, I.V. Zabolotskaya5, O.I. Labetskaya6, A.L. Vorontsov7

1–7 Russian Federation State Scientific Centre – Institute for Biomedical Problems of the Russian Academy of Sciences (Moscow, Russia)

1 a.kochergin_medbio@hotmail.com, 2 dsk1685@gmail.com, 3 andre_markine@mail.ru, 4 juravlyovabc@mail.ru, 5 mikolaz@mail.ru, 6 o.i.labetskaya@rambler.ru, 7 fonbafuta@yandex.ru

The hemostasis system is an important component of homeostatic balance formation in the human body. Clarification of its state under the conditions of a simulated interplanetary flight with the impact on the body of probable emergency situations factors - sleep deprivation, lack of food, water and personal hygiene items, stressful situations associated with a breakdown in communication with the Mission Control Center and intensive operator work, as well as on-planet activities is of great scientific and practical interest. The purpose was to study the effect of physical inactivity with preventive physical activity regime combination as well as simulated stressful situations on the functioning of the hemostasis system within the framework of “SIRIUS-23” experiment with 12-month isolation in a sealed volume. Concentrations of fibrinogen (FBG), plasminogen (PG), D-dimer (DD), antithrombin (ATIII), protein C (PС), as well as values of thrombin time (TT), activated partial thromboplastin time (APTT), and prothrombin time (PT) were measured in the citrate plasma of the volunteer test subjects. On day 61/62 of isolation, a decrease in the concentration of PC and FBG, as well as a lengthening in PT, were detected, which together indicate a decrease in both the pro- and anticoagulant potential of plasma. On day 124/125, a decrease in the FBG level and a lengthening in PT were also noted. On days 186/187 and 250/251, only a lengthening in PT was statistically significant. On day 313/314 and in subsequent periods of the examination, including the recovery period, a lengthening in APTT was observed, indicating a decrease in the potential of the intrinsic coagulation pathway. In addition, on the fifth day of the recovery period, a decrease in the level of PG, FBG and a lengthening in PT were noted, indicating a decrease in the procoagulant potential and fibrinolysis. When examining the blood obtained 10 days later (124-125 days of isolation) after 19 days of limited consumption of food, water, personal hygiene products, as well as the next day (186-187 days of the experiment) after one of the sleep deprivation cycles, no statistically significant changes in the dynamics of most of the parameters studied were found. Only the dynamics of PT showed a reliable stable lengthening from 61/62 to 250/251 days in the range of 4-9%, reflecting a tendency to weaken the coagulation potential along the extrinsic pathway. When comparing the values of the parameters studied before and after imitation of extravehicular activity, a statistically significant increase in the DD level was found on days 130 and 319, which reflects an increase in the intensity of fibrin formation and fibrinolysis processes. The remaining parameters did not change significantly. It was found that the environment of the hermetic object contributes to a decrease in pro- and anticoagulant potential relative to the background level. This effect can be due to the activation of dissimilatory processes in the protein-nucleic acid link of metabolism, as well as the use of a rational complex of physical activities, which together can be considered as a measure for the prevention of thrombotic phenomena in conditions of physical inactivity. The simulated stress situations did not have a noticeable effect on the studied parameters. Simulation of on-planet activity led to an increase in the level of fibrin formation and fibrinolysis activity. The obtained data can be used to develop methods for preventing and correcting the adverse effects of space flight factors on the human body.

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