M.R. Zinurov1, T.V. Bagaeva2, E.E. Zinurova3, M.A. Sysoeva4

1,4 Kazan National Research Technological University (Kazan, Russia)

2 Kazan Federal University (Kazan, Russia)

3 FSAEI НЕ I.M. Sechenov First MSMU of МОН of Russia (Sechenovskiy University) (Moscow, Russia)

1 mihazinurov@gmail.com, 2 tatbag@rambler.ru, 3 lenazinurva@yandex.ru, 4 SysoevaMA@corp.knrtu.ru

The growing demand for lectins in the pharmaceutical, nutraceutical and other industries contributes to the need for their significant growth in the product market. Their functions are diverse. Lectins, including micromycetes, are well known for their role in cell recognition and signaling, as possible modulators of the immune system, and agents involved in the recognition of viral, bacterial, mycoplasma, and parasitic infections. In this regard, there is a need to develop effective schemes for their production.

The kinetics of lectin biosynthesis by micromycetes of the genus Alternaria and the possibility of increasing their activity depending on cell growth and nutrient medium components have been analyzed.

It was found that the synthesis of lectins began during the exponential phase of mycelium growth and increased as the number of dividing cells increased. It was the dividing cells that synthesized lectins. The maximum activity of lectins was observed on days 6-7 of mushroom cultivation, which corresponds to the end of the exponential and the beginning of the stationary growth phase of the micromycete. The active cell division and biomass growth of the fungus Alternaria alternata 4 was facilitated by the introduction of glucose into the nutrient medium. It was on a medium with glucose that the highest titer of lectin activity of these microorganisms (512 units) was recorded. Replacing glucose with a disaccharide, in the form of sucrose, or a polymer, in the form of starch, reduces the titer of lectin activity. A decrease in the titer of lectin activity was also observed in the case of the introduction of micromycete, organic and inorganic nitrogen into the culture medium. If inorganic nitrogen significantly reduced both mycelium growth and the
titer of lectin activity, then the introduction of peptone increased the biomass (by 1.2 times), but the titer of lectin activity was
8-16 times lower compared with the control. An additional addition of amino acids to the nutrient medium: aspartic acid and arginine at a concentration of 50 micrograms/ml, glycine and threonine at a concentration of 100 micrograms/ml contributed to an increase in the titer of Alternaria alternata 4 lectins. However, aspartic acid was preferable because its concentration of 50-100 micrograms/ml increased the activity titer of micromycete lectins by 4 times.

The results obtained can serve as a basis for the development of effective production schemes for the production of active micromycete lectins, with a view to their further practical application.

Zinurov M.R., Bagaeva T.V., Zinurova E.E., Sysoeva M.A. Kinetics of growth and formation of lectins by fungi of the genus Alternaria depending on the time of cultivation and the components of the nutrient medium. Technologies of Living Systems. 2025. V. 22. № 3.
Р. 99-108. DOI: https://doi.org/10.18127/j20700997-202503-10 (In Russian).

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