L.Kh. Pastushkova1, A.G. Goncharova2, D.N. Kashirina3, A.V. Polyakov4, I.M. Larina5

1–5 State Research Center of the Russian Federation –
Institute of Biomedical Problems of the Russian Academy of Sciences (Moscow, Russia)

1 lpastushkova@mail.ru, 2 goncharova.anna@gmail.com, 3 daryakudryavtseva@mail.ru, 5 irina.larina@gmail.com

Considering the decrease in bone mineral density revealed during long-term space flights (SF), it is important to study the molecular mechanisms regulating the state of bone tissue during SF using proteomics methods.

The goal is to determine proteomic markers included in the molecular networks of compensation for morpho-functional changes in the bone system at different times during long-term SF.

A reliable change in proteins participating in adaptive processes occurring in bone tissue was established on the 7th day, 3rd and 6th months of SF. Bioinformatics methods for analyzing the proteome of dried blood spots showed that 11 proteins are associated with biological processes in the bone system on the 7th day of the flight. On the 3rd month, 13 significantly changing proteins are involved in biological processes, of which 6 proteins were identified only in this time period. On the 6th month, 10 proteins were identified, including two new proteins. It should be noted that at each point of the study, all proteins characteristic of the structural and functional adaptation of the skeletal system were linked into a single molecular network. A brief annotation of the functions and significance of each protein is provided.

The data obtained deepen our understanding of the molecular mechanisms accompanying the processes of bone tissue adaptation under spaceflight conditions, including identifying protein-protein interaction networks. This, in turn, allows us to propose certain proteins as potential targets in the program for developing effective measures to prevent skeletal system disorders during long-term space flights.

Pastushkova L.Kh., Goncharova A.G., Kashirina D.N., Polyakova A.V., Larina I.M. Identification of proteomic markers involved in molecular networks of skeletal disorders during long-duration spaceflight. Technologies of Living Systems. 2025. V. 22. № 1. Р. 5-21. DOI: https://doi.org/10.18127/j20700997-202501-01 (In Russian).

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