L.Kh. Pastushkova1, A.G. Goncharova2, A.M. Nosovsky3, D.N. Kashirina4, I.M. Larina5

1–5 SSC RF – Institute of Biomedical Problems of the Russian Academy of Sciences (Moscow, Russia)

1 lpastushkova@mail.ru, 2 goncharova.anna@gmail.com, 3 collega1952@yandex.ru,
4 daryakudryavtseva@mail.ru, 5 irina.larina@gmail.com

It is relevant to obtain data on the modification of the blood proteome during different periods of long-term space flights (SF) and the recovery period. Also relevant is the transition from descriptive statistics to the topology of movement of networks of protein-protein interactions. Using mass spectrometry methods, samples of dried blood spots from 7 Russian cosmonauts were analyzed as part of the “OMICs-DBS” space experiment. The flight duration of the experiment participants was 170–181 days. Taking into account the huge number of isolated proteins and the variability of their levels throughout the SF, an analysis of protein invariance was carried out using the multidimensional scaling method. The cluster of 18 proteins was identified that made connections between the observed variables (the level of isolated proteins) at all observation points before, during and after SF. Thus, for the first time, an attempt has been made to move from descriptive statistics to the topology of the movement of networks of protein-protein interactions during a long space flight and the recovery period after its completion, which makes it possible to evaluate the effect of exposure to space flight factors at different times.

Pastushkova L.Kh., Goncharova A.G., Nosovsky A.M., Kashirina D.N., Larina I.M. Invariance of proteins – participants in biological processes during long-term space flight. Technologies of Living Systems. 2024. V. 21. № 4. Р. 5-15. DOI: https://doi.org/10.18127/ j20700997-202404-01 (In Russian).

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