A.А. Bilyalova1, O.S. Kozlova2, D.D. Filimoshina3, А.I. Bilyalov4, Т.А. Voronina5, G.R. Gazizova6, N.S. Filatov7, А.А. Titova8, K.N. Sultanova9, Е.I. Shagimardanova10, M.V. Vorontsova11, О.А. Gusev12, А.P. Kiyasov13

1–10, 12, 13 Kazan (Volga region) federal university (Kazan, Russia)

4 The Loginov Moscow Clinical Scientific Center Under the Health Department of Moscow (Moscow, Russia)

11 Lomonosov Moscow State University (Moscow, Russia)

11, 12 Endocrinology Research Centre (Moscow, Russia)

12 Graduate School of Medicine, Juntendo University (Tokyo, Japan)

1 alinayakupova96@yandex.ru, 2 olga-sphinx@yandex.ru, 4 BilyalovAir@yandex.ru, 5 vorotaisiya@gmail.com, 6 grgazizova@gmail.com, 7 ns.filatov@yandex.ru, 8 anjerika@list.ru, 9 kasana555_07@mail.ru, 10 rjuka@mail.ru, 11 maria.v.vorontsova@mail.ru, 12 gaijin.ru@gmail.com, 13 kiassov@mail.ru

In connection with the development of gene and cellular methods for the treatment of adrenal diseases, preclinical studies on animal models, usually mice and rats, are required. However, researchers note that the adrenal glands of these animals morphologically and functionally differ significantly from those of humans. In this regard, the question arises: is it advisable to use these animals as a model for studying adrenal pathologies, in particular for evaluating new treatment methods?

The aim of the work is to study the morphological and molecular genetic structure of the adrenal glands of mice of the Acomys.

We morphologically identified three zones of the adrenal cortex of mice of the genus Acomys: zona reticularis, zona fasciculata, zona glomerulosa, which correlates with the structure of the human adrenal gland. We also detected the expression of the CYP17A1 gene, which is present in humans, but is absent in typical laboratory model animals. The protein of this gene is involved in the formation of cortisol and androgens in the adrenal cortex. An annotation of the cell types of the adrenal gland Acomys, obtained by single cell RNA-seq, is presented in this work for the first time.

The results obtained can later be used as a basis for the creation of a new model animal for the study of diseases of the human adrenal gland.

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