E.R. Faskhutdinova1, I.S. Milentyeva2, A.I. Loseva3, L.K. Asyakina4, E.V. Ostapova5

1-5 Kemerovo State University (Kemerovo, Russia)

1faskhutdinovae.98@mail.ru, 2irazumnikova@mail.ru, 3losevaa@mail.ru, 4alk_kem@mail.ru

In the process of aging, there is a decrease in the physiological functions of the body, and, as a result, the development of various diseases. One of the biomarkers of aging is lipofuscin, resulting from lipid peroxidation by reactive oxygen species. Plant extracts rich in biologically active substances of geroprotective action can prevent this process and reduce the accumulation of lipofuscin. The purpose of this study was to study the effect of the plant extract of the Ginkgo biloba callus culture, as well as individual BAS quercetin and kaempferol, on the accumulation of lipofuscin in the body of Caenorhabditis elegans. To measure the accumulation of lipofuscin, the method of spectrofluorimetric measurement and analysis was chosen. The measurements were carried out on a BMG Clariostar spectrophotometer. The objects of the study were the extract of the G. biloba callus culture in 10-fold, 100-fold, and 1000-fold dilutions, as well as quercetin and kaempferol at concentrations of 10, 50, 100, and 200 μM. During the experiment, it was found that the greatest decrease in the accumulation of lipofuscin was demonstrated by the individual BAS quercetin at a concentration of 100 μM. As a result of the impact of this concentration of quercetin on the organism of nematodes, the difference in accumulation decreased by 2,1 times compared with the control. Treatment of nematodes with kaempferol led to a decrease in the difference in the accumulation of lipofuscin by 91% at 50 µM and by 1,3 times at 200 µM. The extract of G. biloba showed a lower efficiency in reducing lipofuscin compared to individual BAS. At the same time, the highest efficiency was observed in a 10-fold dilution of the extract, which reduced the accumulation of lipofuscin by 1.2 times compared with the control.

Thus, individual biologically active substances of plant origin, in particular, quercetin and kaempferol, isolated from the G. biloba callus culture extract inhibit the accumulation of the aging biomarker, which leads to their use as components of dietary supplements or geroprotective functional foods.

Faskhutdinova E.R., Milentyeva I.S., Loseva A.I., Asyakina L.K., Ostapova E.V. Effect of Ginkgo biloba extract and its biologically active substances on lipofuscin accumulation. Technologies of Living Systems. 2023. V. 20. № 4. Р. 121-130. DOI: https://doi.org/10.18127/ j20700997-202304-12 (In Russian).

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