F.V. Donenko1

1 N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of Russia (Moscow, Russia)

With age, the incidence of cancer increases dramatically. There is a hereditary predisposition to the development of oncological diseases. There are no clinical procedures that would preventatively reduce the risk of cancer.

The aim of the work is to publish the results of a study on the effect of the fraction of mouse serum proteins and their administration regimens on the prevention of the growth of hereditary hepatoma of the liver in mice of the CBA line at the age of one and a half years.

Mice of the CDF line at the age of 2-3 months were injected with proteins of the hemoglobin-associated fraction of blood serum. After 1.5 years, mice were investigate and the presence of liver tumors was recorded. It has been shown that prophylactic administration of hemoglobin-associated fraction of mouse serum proteins completely prevents the growth of age-related hepatitis in mice of the CBA line.

This observation is the pilot of clinical prevention of the development of age-related (hereditary) oncological diseases. The fact that the introduction of a certain fraction of blood serum from 5 to 14 times to mice of the CBA line at the age of 2 to 3 months blocks the growth of hepatitis in the same mice at the age of 1, 5 years may be a reason to start such work in humans. The introduction of fractions of human serum proteins is officially allowed. Considering the amount of protein in the injection, the procedure will look like this: one subcutaneous injection for 14 days. The mechanism of this phenomenon can be explained by the fact that the aging of the body is not so much a biological as a physic-chemical process.

Donenko F.V. Prevention of age-related tumor processes by the example of hepatoma growth in mice of the СВА line. Technologies of Living Systems. 2023. V. 20. № 3. Р. 65-72. DOI: https://doi.org/10.18127/j20700997-202303-08 (In Russian).

1. Donenko F.V., Ziganshin R.H., Anisimova N.Y., Voyushin K.E., Sitdikova S.M., Amandzholov B.S., Kiselevskii M.V., Efferth T. Identification of serpin (alpha-1-antitrypsin) as serum growth inhibitory factor in murine ehrlich carcinoma by proteomics. Cancer Genomics Proteomics. 2010. V. 7. № 3. P. 147–156.
2. Donenko F.V., Ziganshin R.K., Sitdikova S.M., Amandzholov B.S., Kiselevskii M.V., Efferth T. Induction of resistance to murine tumor development is associated with alterations in glycosylation of blood serum proteines. Mol. Med. Rep. 2009. V. 2. № 3. P. 487–495. DOI: 10.3892/mmr_00000126
3. Sitdikova S.M., Kiselevskiy M.V., Selchuk V.Yu., Amandzholov B.S., Kurbatova E.A., Donenko F.V. Otsenka effektivnosti immunoterapii kartsinomy yaichnikov myshey SAO-1 Vaktsinami na osnove dendritnykh kletok. Byulleten eksperimentalnoy biologii i meditsiny. 2009. T. 147. № 2. S. 187–189. (in Russian).
4. Donenko F.V., Sitdikova S.M., Kabieva A.O., Moroz L.V. The characteristics of Ehrlich carcinoma recurrence and metastasis. Biull. Eksp. Biol. Med. 1992. V. 114. № 12. P. 652–654.
5. Sitdikova S.M., Amandzholov B.S., Serebryakova M.V., Zhdanovich M.Yu., Kiselevskiy M.V., Donenko F.V. Osobennosti vzaimodeystviya gemoglobina s belkami syvorotki krovi myshey s kartsinomoy Erlikha. Byulleten eksperimentalnoy biologii i meditsiny. 2006. T. 141. № 5. S. 563–566. (in Russian).
6. Donenko F.V., Sitdikova S.M., Syrtsev A.V., Gradyushko A.T., Kiselevsky M.V., Serebryakova M.V., Efferth T. Hemoglobin-associated proteins isolated from blood serum of Еhrlich carcinoma-bearing mice. Int. J. Oncol. 2008. V. 32. № 4. P. 885–893. DOI: 10.3892/ijo.32.4.885
7. Donenko F.V., Sitdikova S.M., Dolzhikova Yu.I., Lebedin Yu.S. Izlecheniye myshey s rasprostranennoy adenokartsinomoy yaichnikov SaO1 s pomoshchyu belkov syvorotki krovi. Byulleten eksperimentalnoy biologii i meditsiny. 2021. T. 172. № 8. S. 2–6. (in Russian).
8. Donenko F.V., Ziganshin R.K., Sitdikova S.M., Amandzholov B.S., Kiselevskii M.V., Efferth T. Induction of resistance to murine tumor development is associated with alterations in glycosylation of blood serum proteines. Mol. Med. Rep. 2009. V. 2. № 3. P. 487–495. DOI: 10.3892/mmr_00000126
9. Kormosh N.G., Davidova T.V., Kopyltsov V.N., Serebryakova M.V., Kabieva A.O., Voyushin K.E., Sitdikova S.M., Amandzholov B.S., Kiselevskii M.V., Donenko F.V. Conformational changes in inter-α-trypsin inhibitor heavy chain 4 activate its tumor-specific activity in mice with B16 melanoma. Mol. Med. Rep. 2015. V. 12. № 3. P. 4483–4493. DOI: 10.3892/mmr.2015.3961
10. Donenko F.V. Faktory. vliyayushchiye na rost opukholi i podkhody k ne tsitotoksichskomu metodu eye lecheniya. Alternativnoye mneniye. Onkoginekologiya. 2021. № 4. S. 4–18. (in Russian).
11. Sitdikova S.M., Amandzholov B.S., Kiselevskiy M.V., Donenko F.V. Spetsifichnost retsidivirovaniya i metastazirovaniya eksperimentalnykh perevivayemykh opukholey kartsinomy Erlikha i melanomy V16. Byulleten eksperimentalnoy biologii i meditsiny. 2007. T. 143. № 1. S. 86–88. (in Russian).
12. Donenko F.V., Kiselevskii M.V. Efferth T. Quantitative regulation of melanoma growth in the host by tumor-specific serpins in blood serum is a main reason for inefficient tumor treatment. Breakthroughs in melanoma research. Ed. Y.Tanaka. Intech, 2011. P. 509-532.
13. Donenko F.V., Kiselewskii M.V., Efferth T. Surgical removal of primary tumors fosters distant metastasis. 7 May 2018. URL: https://stm.sciencemag.org/content/10/436/eaan3464/tab-e-letters