V.N. Shabalin1, S.N. Shatokhina2

1 Research Institute of General Pathology and Pathophysiology (Moscow, Russia)

2 Moscow Regional Research Clinical Institute named after M.F. Vladimirsky (Moscow, Russia)

Blood serum belongs to the class of multicomponent heterogeneous and nonequilibrium media. It is a complex polydisperse system with unstable connections of its components. Blood serum provides humoral informational connections between all the structures of the body, as well as the supply of energy and plastic resources to all vital processes.

Myriads of local metabolic transformations occur simultaneously and consistently in the body. It is quite obvious that all these highly coordinated, coherent changes in the body become possible only through the exchange of information, which must be formed and addressed. This collection, processing and transmission of information, both of an autogenic nature and coming from the external environment, is primarily carried out by serum proteins. Serum proteins perform various functions: they ensure the interaction of cells with each other, determine their survival, stimulate or inhibit growth, differentiation, functional activity and apoptosis, formalize the coordination of actions of the immune, endocrine and nervous systems, carry out active transmembrane transfer of molecules or ions. At the same time, any biological information is structural in nature, and its transmission entails a corresponding change in the molecular structures of the organism.

However, before our research, which began in the 80s of the last century, the concept of morphology (structural analysis) in practical medicine extended only to cellular tissues, and non-cellular tissues - biological fluids, were outside the zone of morphological research. The method cuneiform dehydration of body fluids developed by us provided a methodological basis for studying the structure of non-cellular tissues. The transfer of blood serum to a dehydrated state by this method leads to an integrated, stably ordered form of scattered, rapidly changing structural information that it contains in the native state. At the same time, the processes occurring at the micro- and nanoscale during the transition of blood serum to the solid phase form the corresponding specifics of the structure of macro-level available for visual analysis.

In our theoretical judgments, we adhere to the position that all intra- and intermolecular bonds are of a wave nature. The results of the conducted studies show that the wave rhythms of protein molecules of blood serum in pathological conditions of the body deviate from physiological parameters and change the structure of its facies (structured film of a dehydrated drop). According to the specifics of the structure of the facies of blood serum, it is possible to judge the structural and informational state of the body's proteins, and hence its pathophysiological status as a whole.

The aim of the work is to show the systemic structural and informational role of blood serum in the organization of adaptation mechanisms to the effects of autogenic and exogenous factors. The video materials of solid-phase structures (facies) of human blood serum taken from the database formed by us during 2001-2021 were studied. More than 20 thousand microphotographs and more than 100 micro videos were analyzed. The results of the conducted studies have shown that the facies structure reflects the structural features of serum protein molecules and makes it possible to determine both physiological and pathological conditions of the body, as well as to identify the nature of the action of both chemical and physical factors of treatment. As a result, we get fundamentally new information that is available for diagnosis in a wide clinical practice.

Shabalin V.N., Shatokhina S.N. Blood serum as an information and analytical system of the human body. Technologies of Living Systems. 2023. V. 20. № 1. Р. 36-45. DOI: https://doi.org/10.18127/j20700997-202301-04 (In Russian)

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