A.A. Cheremiskina¹, V.M. Generalov², A.S. Safatov³, G.A. Buryak4, A.L. Aseev5

1–4 Federal State Research Institution State Research Center of Virology and Biotechnology «Vector» Rospotrebnadzor     (Koltsovo, Novosibirsk Region, Russia)

5 Institute of Semiconductor Physics named after A.V. Rzhanov, Siberian Branch of the Russian Academy of Sciences

Novosibirsk State University (Novosibirsk, Russia)

Surface preparation of a biosensor based on a silicon nanowire field-effect transistor is one of the main problems of creating a highly selective and sensitive analytical device. It consists in cleaning the surface from possible numerous organic and inorganic contaminants. Currently, there are a large number of methods of purification, but their choice is still relevant in solving specific problems (for example, in determining the nomenclature of solutions used, the quality of chemical reagents, their concentration, the degree of their own purity). It is obvious, that poorly performed cleaning or incorrectly selected conditions for its implementation, finally, affect the effectiveness of the immobilization of biological material on the surface of the biosensor and its performance indicators as a whole. The goal of this work is to review the literature data on the methods of preparing the substrate surface of silicon nanowire field-effect transistors.

The paper collects and systematizes information concerning the classification of methods for cleaning the surface from organic and inorganic contaminants, options for performing the procedure for immobilizing biological material. Based on the requirements imposed on them, the main advantages and disadvantages are considered. As a result of the analysis, the most popular methods of preparing the surface of the biosensor are noted, according to the authors of this work.

The article presents an opportunity to familiarize the reader with the problem of preparing a biosensor based on a silicon nanowire field-effect transistor, the formulation of unresolved issues in this field of research, as well as the study of existing achievements. The presented review can be used to: develop and create more advanced approaches for cleaning and preparing the substrate surface of silicon nanowire field-effect transistors; evaluate the generalized level of laboratory sample preparation and predict the further development of sample preparation; lecture courses on the problems of biotechnology in the specialty 03.01.06-Biotechnology (including bionanotechnology).

Cheremiskina A.A., Generalov V.M., Safatov A.S., Buryak G.A. Preparation of the substrate surface of silicon nanowire field-effect transistors to create a biosensor. Technologies of Living Systems. 2021. V. 18. № 2. Р. 62–70. DOI: https://doi.org/10.18127/j20700997202102-08 (in Russian).

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