A.A. Markin – 

Ph.D. (Med.), Associate Professor, Head of Laboratory of medical Biochemistry and Neuroendocrinology,  Russian Federation State Scientific Centre – Institute for Biomedical Problems of the RAS (Moscow)  E-mail: andre_markine@mail.ru

О.А. Zhuravleva – 

Ph.D. (Med.), Leader Research Scientist, Laboratory of Medical Biochemistry and Neuroendocrinology Russian Federation State Scientific Centre – Institute for Biomedical Problems of the RAS (Moscow) E-mail: juravlyovabc@mail.ru

D.S. Kuzichkin – 

Ph.D. (Biol.), Senior Research Scientist, Laboratory of Medical Biochemistry and Neuroendocrinology,  Russian Federation State Scientific Centre – Institute for Biomedical Problems of the RAS (Moscow) E-mail: dmitry161985@mail.ru

T.A. Smirnova – 

Ph.D. (Med.), Leader Research Scientist, Office of the Academic Councils and Postgraduate Study, 

Russian Federation State Scientific Centre – Institute for Biomedical Problems of the RAS (Moscow) E-mail: smirnova@imbp.ru

The purpose of this study was to study the peculiarities of metabolic reactions in volunteers with 17-day isolation in the hermetic chamber. The experiment involved 6 volunteers: three women aged 27–37 years and three men aged 33–43 years who made up two groups formed by gender. The values of 48 biochemical parameters reflecting the state of myocardium, liver, gastrointestinal tract, kidneys, muscular and bone systems, peculiarities of protein, carbohydrate, lipid, electrolyte, mineral, pigment, nucleic and energy metabolism were studied in the blood of the participants of the experiment. Venous blood was taken for analysis 7 days before the start of isolation (background), on the 7th and 14th day of exposure, as well as on the 2nd and 7th day of the recovery period (RP).

In the group of men on the second day of RP the signs of stress-reaction and hypohydration were found: the content of nonesterified (free) fatty acids (NEFA) was increased beyond the reference interval, the concentration of chlorides was increased, the level of sodium and phosphorus was higher than the physiological norm. The reason for this may be the effects of stress reactions associated with simulated emergency situation, 48-hour sleep deprivation.

In women, starting from 14 days of the experiment to the seventh day of the recovery period, a complex of changes was observed, characterizing the development of stress reaction with the mobilization of energy-synthetic systems and hypohydration of the body. This could be due to completion of the luteal phase of the menstrual cycle in two participants of the experiment. Significantly beyond the physiological norm increased activity of lactate dehydrogenase, elevated glucose level, increased concentration of NEFA and phospholipids, increased the value of the atherogenic index. Increased levels of calcium, magnesium, phosphorus, uric acid, creatinine, clinically significant – chlorides. Within the normal range, the total protein and albumin content increased. In women, the level of serum iron decreased, the concentration of urea increased, which is associated with the onset of the menstrual phase of the cycle.

Thus, the short-term influence of a complex of factors of a hermetic volume does not lead to the formation of metabolic shifts observed in similar experiments of long duration. Leading factors in the development of the changes found were nonisolation effects – simulated emergency situations, dietary habits, work and rest schedules. A certain role is played by gender differences.

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