G.I. Myandina - Dr. Sc. (Biol.), Professor, Department of Biology and General genetics, Peoples - Friendship University of Russia, Moscow E-mail: gmyand@mail.ru E.V. Tarasenko - Ph.D. (Biol.), Associate Professor, Department of Biology and General genetics, Peoples - Friendship University of Russia, Moscow E-mail: tarasenko_ev@inbox.ru L.A. Varekha - Post-graduate Student, Department of faculty therapy, Peoples - Friendship University of Russia, Moscow E-mail: varekha@mail.ru

The results of the bioinformatics analysis of the sequences of the retrovirus type D chronically infected human pharynx carcinoma cells have confirmed its similarity to monkey virus SRV-1. The nucleotide sequences of integrated provirus has been characterized and compared with SRV-1 virus genome. We have detected a couple non-significant single-nucleotide substitutions in the genome of this virus integrated in human DNA. These substitutions do not change the amino acid se-quence in translated protein or slightly change it without prevention of the functional activities. The variability of the re-trovirus DNA integrated in human genome is studied and possible mechanism of integration of retroviral DNA in non-permissive host genome is suggested.

 

1. Itkes A.V., Mjandina G.I., Gurjanova O.I., Sivov I.G., Galaktionova T.S., Lebedev JU.B. Integracija DNK retrovirusov obezjan v khromosomy cheloveka // Russkijj zhurnal «VICH/SPID i rodstvennye problemy». 2004. T. 8. № 2.  S. 25.
2. Lebedev JU.B., Bolorma V., Kzhishkovska JU.G., Ostashkin A.S., Ilin K.V., Mjandina G.I., Pjagajj P.EH., Itkes A.V. Integracija genoma retrovirusa tipa D Mejjzona-Pfajjzera v khromosomu cheloveka // Molekuljarnaja biologija. 2002. T. 36. № 6. S. 1012-1014.
3. Mjandina G.I., Gurjanova O.I., Pjagajj P.EH., Itkes A.V, Lebedev JU.B. Kompjuternyjj analiz sajjta integracii retrovirusa tipa tipa D Mejjzona-Pfajjzera v khromosomy cheloveka // Vestnik RUDN. Ser. Medicina, Genetika. 2003. № 5. S. 41-46.
4. Mjandina G.I. Analiz integrirovannogo retrovirusa SRV-1 obezjan v DNK cheloveka // Tekhnologii zhivykh sistem. 2012. T. 9. № 7. S. 30-33.
5. Morozov V.A., Lagaye S., Lyakh L., Meulen J. Type D retrovirus markers in healthy Africans from Guinea // Res. Virol. 1996. V. 147. P. 341-351.
6. Ford R.J., Donehover L.A., Bohannon R.C. Studies on a type D retrovirus isolated from an AIDS patient lymphoma // AIDS Res. Hum. Retroviruses. 1992. V. 8. P. 742-751.
7. Ilyin KV, Kzhyshkowska JG, Imamova LR, Mashkova TD, Ostashkin AS, Kiselev AV, Gordina GA, Kurmashow VI, Itkes AV. Type D retrovirus specific sequences in lymphocytes of the children with Burkitt-type lymphoma and their parents // Immunol Lett. 2001. V. 78. P. 51-54.
8. Lerche N.W., Switzer W.M., Yee J.L., et al. Evidence of infection with simian type D retrovirus in persons occupationally exposed in nonhuman primates // Journal of Virology. 2001. V. 75. N. 4. P.492-497.
9. Mansky L.M. In vivo analysis of Human T-cell Leukemia virus type 1 reverse transcription accuracy // Journal of Virology. 2000. V. 74. № 20. P. 9525-9531.
10. Sakalian M., Rapp N.D. Rescue of internal scaffold-deleted Mason-Pfizer monkey virus particles production by plasma membrane targeting // Virology. 2006. V. 345. № 2. P. 317-327.
11. Woolhouse M.E.J., Taylor L.H. Population biology of multihost pathogens // Science. 2001.  V. 292. P. 1109-1112.