350 rub
Journal Technologies of Living Systems №8 for 2016 г.
Article in number:
The role of direct contacts between mesenchymal stromal cells and endo-thelial cells in vascular-like structures formation in vitro
Keywords: urokinase receptor connexins mesenchymal stromal cells of adipose tissue endothelial cells cell-cell inte-ractions angiogenesis
Authors:
N.V. Koptelova - Therapeutist, City clinical hospital № 64 E-mail: koptelovan@gmail.com I.B Beloglazova - Ph.D. (Biol.), Research Scientist, Angiogenesis laboratory, Russian Cardiology Research and Production Complex, Moscow E-mail: irene@cardio.ru E.S. Zubkova - Junior Research Scientist, Russian Cardiology Research and Production Complex, Moscow E-mail: zubkova@cardio.ru O.Y. Sukhareva - Ph.D. (Med.), Leading Research Scientist, Endocrinology Scientific Center, Moscow D.T. Dyikanov - Post-graduate Student, Laboratory Assistant, Faculty of Fundamental Medicine, Lomonosov State University E-mail: danidy@inbox.ru E.I. Ratner - Ph.D. (Biol.), Senior Research Scientist, Russian Cardiology Research and Production Complex, Moscow E-mail: eiratner@gmail.com Z.A. Akopyan - Ph.D. (Med.), Academic Research Secretary, Faculty of Fundamental Medicine, Lomonosov Moscow State University M.V. Shestakova - Dr.Sc. (Med.), Professor, Corresponding Member RAS, Endocrinology Scientific Center, Moscow Y.V. Parfyonova - Dr.Sc. (Med.), Professor, Head of Angiogenesis Laboratory, Russian Cardiology Research and Production Complex; Head of Postgenomic Medical Technology Laboratory, Faculty of Fundamental Medicine, Lomonosov Moscow State University E-mail: veparfyon@mail.ru
Abstract:
The aim of this work is to study the molecular mechanisms of interaction between mesenchymal stromal cells (MSC) with endothelial cells (EC) during the formation of the vasculature. It was found that in co-cultivation of EC with MSC on the pure glass only direct contacts between EC and MSC promote the formation of vascular like structures by EC. Using flow-cytometry it was shown that co-cultivation cells in direct contacts increased urokinase receptor expression on the surface of EC. This increase is responsible for vascular structures formation because it is suppressed in dose dependent manner by the inhibition of urokinase receptor by specific antibodies. Using RT-PCR it was shown that the co-cultivation caused the upregulation of urokinase system genes - urokinase and PAI-1. Cell interaction through connexins is also partly responsible for the vascular like structures formation in co-cultivation of MSC and HUVEC as connexin inhibitors reduced the length of these structures by 25 %.
Pages: 4-13
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