O.V. Astafyeva - Ph.D. (Biol.), Assistant Professor, Department of Biotechnology and Bioecology, Astrakhan State University. E-mail: astra39@list.ru D.D. Vilkova - Master-s Student, Department of Biology, Astrakhan State University. E-mail: easy_breath@mail.ru L.T. Sukhenko - Dr.Sc. (Biol.), Associate Professor, Professor, Department of Biotechnology and Bioecology, Astrakhan State University. E-mail: sukhenko@list.ru D.K. Magzanova - Ph.D. (Biol.), Senior Research Scientist, Laboratory of Biotechnology, Astrakhan State University. E-mail: dmagzanova@mail.ru Yu.V. Bataeva - Ph.D. (Biol.), Senior Research Scientist, Laboratory of Biotechnology, Astrakhan State University. E-mail: aveatab@mail.ru M.A. Egorov - Dr.Sc. (Biol.), Professor, Head of Department of Biotechnology and Bioecology, Astrakhan State University. E-mail: egorovs.mail@gmail.com

In research work we evaluated the influence of steroid phytohormones epibrassinolide on the development of certain mi-croorganisms. As the test objects nonpathogenic strains were selected - S. aureus, E. coli and B. subtilis, the culture of B. megaterium extracted from the rhizosphere of bean plants, culture of yeast S. cerevisiae. The influence of epibrassinolide on bacteria was tested by method of serial dilutions in liquid medium. We measured turbidity (light transmittance T%) of solutions by photometric method at a wavelength of 600 nm on a digital photoelectric colorimeter AR-101 (APEL, Japan). After 24 hours of development of bacterial cells we made bacterial preparations from suspensions which were stained by the Gram-s method and were microscoped. We also studied the influence of epibrassinolide on the development of yeast by method of bacterial inoculation of yeast in liquid medium where the sample of epibrassinolide of various concentrations was added. After 24 hours of exposure at 28 C were evaluated morphological properties, the composition and reproduction of the yeast cells influenced by epibrassinolide and in a control sample. As a result of experiments it was found that the mother liquor of the epibrassinolide preparation of concentrations 1 µg/ml had an inhibitory effect expressed in different degrees on the growth and development of E. coli, S. aureus, B.subtilis, B. megaterium. Culture of gram-negative bacteria E. coli was shown as more sensitive to the action of epibrassinolide. The concentration of 0.1 µg/ml had the best antibacterial effect on B. subtilis, B. megaterium and E. coli. Concentration of 0.01 µg/ ml showed inhibitory effect on the growth and the development of B.megaterium. Solution of epibrassinolide in concentration of 0.01 µg/ml had a stimulating effect on the growth and development on S. aureus. Epibrassinolide in concentration of 0.001 µg/ml had a stimulating effect on the development of culture B. subtilis. A study of morphological properties of selected bacteria before and after exposure to epibrassinolide showed the change in size and form of cells. The sample of epibrassinolide in concentration of 0.02 µg/ml had stimulatory effect on the cells of yeast while the concentrations of 0.005 and 0.01 µg/ml had no noticible effect. Thus the preparation of steroid phytohormone epibrassinolide in different concentrations can be used as an antibacterial agent and as a stimulant for the growth of certain beneficial microorganisms. For example, you can use the preparation of epibrassinolide for seed treatment to protect them from infection, to accelerate growth of soil bacterium B.megaterium which is used in preparations for stimulation of plant growth and to stimulate the growth of baker's yeast.

 

1. Dubinina I.M., Burakhanova E.A. Dejjstvie ehpibrassinolida na fotosinteticheskuju aktivnost i obrazovanie 14S produktov fotosinteza u Beta vulgaris L. // V Mezhdunar. konf. «Reguljatory rosta i razvitija rastenijj». M. 1999. S. 90.
2. Egorov M.A. Fiziologicheskie osobennosti dejj­stvija fitogormona ehpibrassinolida na organizm zhivotnykh v rannem ontogeneze. Astrakhan: Izdat. dom «Astrakhanskijj universitet». 2007. 248 s.
3. Brosa C. Biological effects of brassinosteroids // Crit. Rev. Biochem. Mol. Biol. 1999. № 34.  P. 339-358.
4. Sasse J.M. Physiological actions of brassinosteroids: an update // J. Plant. Growth. Regul. 2003. №22. P. 276-288.
5. Runkova L.B., Talieva M.N., Aleksandrova B.C. Vlijanie ehpibrassinolida i preparatov na ego osnove na ukorenenie, rost i razvitie dekorativnykh rastenijj // Polifunkcional­nost dejjstvija brassinosteroidov. M.: NEHST 2007. S.293-299.
6. Kalimullina I. Vlijanie EHpina-EHkstra na urozhajj s/kh kultur // NEHST-M, publikacii, 2014. Rezhim dostupa: http://www.nest-m.ru/index.php/publikatsii/stati-o-preparatakh /regulyatory-rosta/epin-ekstra/404-vliyanie-epi­na-ekstra-na-urozhaj-s-kh-kultur.html.
7. PshenichnayaL.A., KhripachV.A., VolynetzA.P., ProkkhorchikR.A., ManzhelesovaN.E., MorozikG.V.  Brassinosteroids and resistance of barley plants to leaf diseases // In: Parfenov V.I. (Ed.) Problems of Experimental Botany. Minsk: Byelorussian Science. 1997. P. 210-217.
8. Egorov M.A. The influence of phytogormone epibrassinolid on the vertebral animals in the early ontogenesis // Biotechnology Titles:  Biotechnology and agriculture and the food industry. USA. New York State: Nova science publishers, Inc. 2004. P. 65-67.
9. Roth U., Friebe A., Schnabl H. Resistance induction in plants by a brassinosteroid-containing extract of Lychnis Viscaria L. Z. Naturfor. C-A. // J. Biosci. 2000. №55. P. 552-559.
10. Nakashita H., Yasuda M., Nitta T., Asami T., Fujioka S., Arai Y., Sekimata K., Takatsuto S., Yamaguchi I., Yoshida S. Brassinosteroid functions in a broad range of disease resistance in tobacco and rice // Plant J. 2003. №33. P. 887-898.
11. Zhu Z., Zhang Z., Qin G., Tian S. Effects of bras­sinosteroids on postharvest disease and sense­cence of jujube fruitin storage // Postharvest. Biology and Technology. 2010. №56. P. 50-55.
12. Borisova T. Novaja paradigma dlja proizvo­ditelejj selskokhozjajjstvennojj produkcii // NEHST-M, publikacii, 2013 - URL: http:// www.nest-m.ru/index.php/ publikatsii/stati-o-preparatakh/177-novaya-paradigma-dlya-proiz­voditelej-selskokhozyajstvennoj-produktsii.html.
13. Egorov M.A. Antioxidant Action of Biologically Active Substance of Brassinosteroids Class - Phytohormone Epibrassinolide // Biotechnology in Medicine, Foodstuffs, Biocatalysis, Environ­ment and Biogeotechnology. USA.New York State:Nova science publishers, Inc. 2010.  P. 23-31.
14. CHan Min Kuan, Egorov M.A., Bataeva JU.V. Roststimulirujushhijj ehffekt shtamma Basillus megaterium v vegetacionnom opyte // Vest­nik Altajjskogo gosudarstvennogo agrarnogo universiteta. 2012. № 3 (89). S. 46-49.
15. Dzerzhinskaja I.S. Metody vydelenija, issledovanija i opredelenija antibioticheskojj aktivnosti mikroorganizmov, obladajushhikh antago­nisticheskimi svojjstvami: Metodicheskie ukazanija. Astrakhan: AGTU. 2005. S. 54-64.
16. Lakin G.F. Biometrija: Uchebnoe posobie dlja biol. spec. vuzov, 4-e izd., pererab. i dop. M.: Vysshaja shkola. 1990. 352 s.
17. Vyzulin S.A., Vyzulina V.I. Zavisimost skorosti rosta khlebnykh drozhzhejj ot prodol­zhitelnosti obluchenija sverkhvysokochas­totnym magnitnym izlucheniem // Materialy Mezhdunar. distancionnojj nauchn.-prakt. konf. «Processy i javlenija v kon­densirovannykh sredakh» (Krasno­dar, 27 sentjabrja 2004). Krasno­dar: Izd-vo Kuban. gos. unta. S. 176-181.
18. Smashevskijj N.D. Antivitamin pantotenovojj kisloty (pizamin) v vysshem rastenii (biolo­gicheskaja rol i mekhanizm dejjstvija): Mono­grafija. Astrakhan: Izd. dom «Astrakhanskijj universitet». 2008. 254 s.