S.L. Sokolova - Ph.D. (Med.), Senior Lecturer, Department of Biology and General Genetics, Peoples - Friendship University of Russia (Moscow). E-mail: sokolova_sl@pfur.ru N.I. Buyanova - Ph.D. (Med.), Senior Lecturer, Department of Biology and General Genetics, Peoples - Friendship University of Russia. E-mail: buyanova_ni@pfur.ru E.M. Zheludova - Ph.D. (Biol.), Associate Professor, Department of Biology and General Genetics, Peoples - Friendship University of Russia. E-mail: zheludova_em@pfur.ru

The ability of cells Escherichia coli AP132 to create polycellular forms under the influence of different factors was investigated. We used cells E. coli without plasmids and cells, containing F-like plasmids рАР10-2 and рАР41, that had been marked by us earlier with transposons Tn9 and Tn1, and bacteriophage MS2 is valid to adsorb specifically on «sex» pili. Polycellular forms of bacteria were detected by the method of low-angle laser radiation scattering («Malvern 3600 Ec»). The functional activity of conjugative pili synthesized under genetic control of F-like plasmids is the essential factor, which determine the creation of bacterial polycellular forms. For the first time the role of pili-specific bacteriophage MS2 in the formation of cell associates was shown. Polycellular forms are able to enhance considerably the level of bacterial drug resistance. It should be considered during developing of the strategy of rational antibiotic therapy of infectious diseases of human and animals.

 

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