S.V. Nadezhdin - Ph.D. (Biol.), Associate Professor, Department of Ecology, Physiology and Biological Evolution, Federal State Autonomous Educational Institution of Higher Professional Education «Belgorod National Research University». E-mail: nadezhdin@bsu.edu.ru V.V. Sirota - Ph.D. (Phys.-Math.), Director of Centre for Construction Ceramics and Engineering Prototyping, Research and Development Centre, Federal State Autonomous Educational Institution of Higher Professional Education «Belgorod National Research University». E-mail: sirota@bsu.edu.ru M.G. Kovaleva - Ph.D. (Phys.-Math.), Senior Research Scientist, Centre for Collective Use of Scientific Equipment «Diagnostics of structure and properties of nanomaterials» of Belgorod National Research University, Federal State Autonomous Educational Institution of Higher Professional Education «Belgorod National Research University». E-mail: kovaleva@bsu.edu.ru I.A. Pavlenko - Student, Faculty of Engineering and Physics of Institute of Engineering Technology and Natural Science, Federal State Autonomous Educational Institution of Higher Professional Education «Belgorod National Research University». E-mail: dancer.disco@mail.ru E.V. Zubareva - Ph.D. (Biol.), Senior Lecturer, Department of Ecology, Physiology and Biological Evolution, Federal State Autonomous Educational Institution of Higher Professional Education «Belgorod National Research University». E-mail: zubareva-e@yandex.ru A.N. Solov'eva - Student, Faculty of Biology and Chemistry, Institute of Engineering Technology and Natural Science, Federal State Autonomous Educational Institution of Higher Professional Education «Belgorod National Research University». E-mail: aleon.solowiewa2014@ya.ru

The research was carried out to estimate the efficiency of the usage of magnesium carbonate obtained from natural amorphous magnesite (Khalilovsky deposit, Orenburg Region, Russia) as a source of alimentary magnesium to eliminate of hypomagnesemia. During experiments animals (20 male Wistar rats weighting about 250-300 grams) were randomly divided into two groups: the control and the experimental one containing 10 animals per each. The animals of the control group were kept on a standard ration in the vivarium with free access to the potable water. The rats from the experimental group were kept on a magnesium-free diet and used distilled water during the first month of the experiment. Magnesium-free diet was made with regard to existing diet produced by the company ICN Biomedicals Inc. (Aurora, Ohio, USA) [1, 2]. Upon the expiry of the first month the blood samples from the experimental animals were taken to detect of magnesium in the blood, determine the leukogram and count the total number of the white blood cells. The blood drop was put on the glass slide, dried on the air and placed in the chamber of scanning electron microscope Quanta 200 3D (FEI) for the electron probe microanalysis with use of the attachment TEAM EDS (EDAX). Leukogram was determined by counting the cells in the stained blood smears which had been made by established procedure. Total number of leukocytes in 1 mm3 of blood was calculated with the use of Goryaev chamber. One month later the rats from the experimental group began to receive the magnesium-containing solutions (MgCO3+Н2О) through the feeding tube at 50 mg of elementary magnesium per 1 kg of the animal weight. At the end of the second month the animals from the experimental group were subjected to the blood spot sampling from the tail vein for detection of increase in the magnesium level in the blood. It was determined that developed diet caused the magnesium deficiency in the peripheral blood and could be used to simulate hypomagnesemia in small laboratory animals. It was revealed that keeping the rats of the experimental group on the magnesium-free diet led to decrease in the magnesium content in the peripheral blood by 6,41 percent in comparison with the control group. Peroral entry of magnesium (MgCO3+Н2О) during the second month caused the increase of its level in the peripheral blood by 3,58 percent in comparison with the period of magnesium-free diet. But the reached level of magnesium remained below than value of the control group by 2,83 percent. It was observed that after introduction of magnesium carbonate to the diet of the animals of experimental group the activation of reactive body state had been registered which described by the shift of leukogram to the left indicating the overstress of regulatory body systems.

 

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