V.V. Rozanov - Dr.Sc. (Biol.), Ph.D. (Phys.-Math.), Professor, Leading Research Scientist, Physical Faculty, Lo-monosov Moscow State University; Head of Laboratory of Scientific and Educational-Methodic Centre Bio-Medical Technologies, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants, Moscow, Russia. E-mail: vrozanov@mail.ru I.V. Matveychuk - Dr.Sc. (Biol.), Professor, Head of Scientific and educational-Methodic Centre Bio-Medical Technologies, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants, Moscow, Russia. E-mail: nizbmtvilar@mtu-net.ru M.V. Lekishvili - Dr.Sc. (Med.), Head of Laboratory «Tissue Bank», N.N. Priorov Research Institute of Trauma-tology and Orthopaedics (CITO), Moscow, Russia. E-mail: leki@mail.ru Y.Y. Litvinov - Deputy Head, Scientific and Educational-Methodic Centre of Bio-Medical Technologies, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants, Moscow, Russia. E-mail: nizbmtvi-lar@mtu-net.ru T.M. Andreeva - Post-graduate Student, Department of Accelerators Physics and Radiation Medicine, Physical Faculty, Lomonosov Moscow State University, Moscow, Russia. E-mail: andreevatm@mail.ru А.А. Nikolaeva - Post-graduate Student, Department of Accelerators Physics and Radiation Medicine, Physical Faculty, Lomonosov Moscow State University, Moscow, Russia. E-mail: naa90@mail.ru

The purpose of the study  development and implementation in practice of the tissue bank work innovative technolo-gies combined sterilization of bone implants. Due to the increase of injuries, the number of post-traumatic and post-operative complications is growing and the need of replace the plastic material of various defects. The dynamics of this process can be clearly illustrated by the following figures: in 1992 in the US was transplanted allogenic bone about 200,000, and in 2002 this figure reached 800,000 transplants. Moreover, this situation is typical of most developed countries. Thus, even the most modest es-timates show that today the need of allogenic bone graft is not counted in hundreds of thousands and millions per year. This infection of the recipients of such operations is a critical concern for their safety. The difficulties are exacerbated by a general weakening of the immune system of patients. One of the ways to solve this problem is the development and improvement of technologies implants - sterilization, the development of new and innovative approaches. Radiation sterilization using gamma rays or fast electrons flow is considered today one of the most effective methods. This technology is increasingly used in tissue banks in Russia, Japan, the US, China, EU, it provides a low-temperature sterilization treatment of implants to a few centimeters thick. Wherein the bone fragments are placed in a sealed package to prevent secondary infection. However, the technology is not free from several problematic issues. And most important of these is the absorbed dose. As a standard sterilization dose now considered to be the value of 25 kGr. However, as shown by numerous experiments, complete the uniqueness does not exist here. The method of radiation sterilization must satisfy two competing requirements: on the one hand, the reported dose should be large enough to provide a reliable bactericidal effect on microorganisms with different radiosensitivity. On the other hand, upon exposure to sterilizing radiation bone implant should maintain high osteoinductive ability, and this dosage should be minimized as much as possible. The situation is complicated by the fact that the effectiveness of the sterilization process of bone implants depends not only on the possible infection of donor material, but also on many other parameters, such as initial seeding of bone fragments, the conditions for their pre-storage modes of sterilization treatment. As a result of the high efficiency of the radiation sterilization can only be achieved by using new combination of innovative technologies, which are based combined effect of a number of physical and chemical factors that can help to account for the effect of most of these parameters.