V.V. Melekhov - Post-graduate Student, FSSI Institute for Analytical Instrumentation RAS (Pushchino, Moscow Region). E-mail: maintain@inbox.ru G.A. Enin - Senior Electronic Engineer, FSSI Institute of Protein Research RAS (Pushchino, Moscow Region). E-mail: yenin@vega.protres.ru V.B. Gavriliouk - Ph.D. (Chem.), Senior Research Scientist, FSSI Institute of Theoretical and Experimental Biophysics RAS (Pushchino, Moscow Region). E-mail: vbg@mail.ru B.K. Gavriliouk - Dr.Sc. (Biol.), Professor A.A. Timchenko - Ph.D. (Phys.-Math.), Research Scientist, FSSI Institute of Protein Research RAS (Push-chino, Moscow Region). E-mail: atim@vega.protres.ru

In this work the dependence of collagen matrix structure was investigated by atomic force microscopy upon changes of collagen concentration, pH of medium and its temperature. AFM measurements demonstrated the stability of the net structure of collagen matrix upon dilution up to concentration of collagen of 0.3 mg/ml. It was found that the net structure of matrix is stable at acidic pH up to pH 6. It was observed that the net structure is stable at temperatures up to 370C where at 370C its structure is more uniform. The obtained results open new possibilities of optimization of healing sample preparation on the basis of collagen and raise efficiency of their application in medicine.

 

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