A.A. Skalny - Physician, ANO «Centre for Biotic Medicine», Moscow. E-mail: andrey_sk@microelements.ru A.A. Tinkov - Ph.D. (Med.), Assistant, Department of Biochemistry, Orenburg State Medical University, Orenburg, Russia; Researcher, Laboratory of Biotechnology and Applied Bioelementology, Yaroslavl State UniversityYaroslavl, Russia. E-mail: tinkov.a.a@gmail.com Yu. S. Medvedeva - Research Scientist, Laboratory of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia I.B. Alchinova - Research Scientist, Laboratory of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia O.P. Ajsuvakova - Ph.D. (Chem), Assistant, Department of Chemistry, Orenburg State Pedagogical University, Orenburg, Sovetskaya. E-mail: oajsuvakova@gmail.com E.Yu. Bonitenko - Dr.Sc. (Med.), Professor, Director of Federal State Scientific Institution «Institute of Toxicology», Federal Medico-Biological Agency, St. Petersburg, Russia M.Yu. Karganov - Dr. Sc. (Biol.), Professor, Head of Laboratory of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia. E-mail: mkarganov@mail.ru A.A. Nikonorov - Dr. Sc. (Med.), Professor, Head of Department of Biochemistry, Orenburg State Medical University, Orenburg, Russia. E-mail: nikonorov_all@mail.ru M.G. Skalnyay - Dr.Sc. (Med.), Professor, Directir of ANO «Centre for Biotic Medicine», Moscow Yu.N. Lobanova - Ph.D. (Biol.), Deputy Head of Department of Laboratory of LLC «Micronutrients»

Determination the influence of Zn on the distribution of toxic trace elements in the body. Methods: The content of Zn and toxic trace elements in the liver, kidney, heart, skeletal muscle, serum and hair of Wistar rats by the daily intragastric administration of zinc (II) asparaginate (Zn (C4NO4H6) 2 ∙ Zn (OH) 2) in doses of 5 and 15 mg / kg for 7 and 14 days was studied. The animals received ad libitum diet standard PC-120 for the maintenance of laboratory animals (LLC "Laborator-korm", Moscow) with a total energy value of 307 kcal / 100 g and the determine content of the metals. After removing the animals from the experiment, the samples of the liver, kidneys, heart, calf serum, and animal hair were extracted. The chemical analysis of the samples (determination of Zn, Al, As, Li, Ni, Pb, Sn, Sr) was carried out by mass spectrometry with inductively coupled argon plasma on the device NexION 300D (PerkinElmer Inc., Shelton, CT 06484, USA), equipped with automatic dispenser ESI SC-2 DX4 in compliance with the manufacturer's instructions and the use of standard solutions of trace elements and reference samples. Results: intragastric administration of zinc (II) asparaginata leads to a change in the content of the metal in the organs and tissues of animals with more significant changes to the 14th day of the experiment in relation to the dosage of 15 mg / kg. In the muscle tissue and wool no any changes of Zn content were observed in all stages of the experiment. Changing the content of toxic metals and metalloids had multidirectional nature. Thus, reducing the Al content was observed in the liver, muscle and wool, Li - in muscle, Ni - in the liver, kidney, heart, muscle and wool, Pb - in muscle, Sn - in the liver and serum, Sr - in the kidney, heart and wool. Elevated levels of As was found in the heart and serum, Li - in the kidney and heart (day 7), Pb- in heart.Conclusion: The results of this study showed redistribution of toxic metals in the body of animals under the influence of zinc (II) asparaginate entrance. At the same time, more research is needed to clarify the nature and study the mechanisms of the observed effects.

 

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