R.V. Shafigulin - Ph.D. (Chem.), Associated Professor, Department of Physical Chemistry and Chromatography, Samara State University. E-mail shafiro@mail.ru Z.E. Mashchenko - Ph.D. (Pharm.), Associated Professor, Department of Technology of Food roduction and Perfumes and Cosmetic Products, Samara State Technical University. E-mail mzinaida@yandex.ru M.V. Somova - Magistr, Department of Physical Chemistry and Chromatography, Samara State University. E-mail mascha.somova2010@yandex.ru A.V. Bulanova - Dr. Sc. (Chem.), Professor, Department of Physical Chemistry and Chromatography, Samara State University. E-mail av.bul@yandex.ru I.F. Shatalaev - Dr. Sc. (Biol.), Professor, Head of the Chemistry Department of Pharmaceutical Faculty, the Chemistry Department of Pharmaceutical Faculty, Samara State Medical University. E-mail shatalaev@list.ru P.G. Mizinа - Dr. Sc. (Pharm.), Professor, FGBNU «All-Russian Research Institute of Medicinal and Aromatic Plants». E-mail mizina-pg@yandex.ru

Cefazolin is a broad - spectrum antibiotic of cephalosporin group, often used in medical practice and found in the process water plants for the production of antibiotics, urban wastewater [1, 2]. The structural formula is anticipated presented in Fig. 1. The most sensitive analytical method for the determination of Cefazolin is a high-performance liquid chromatography (HPLC), which is often used for environmental control of the content of high molecular weight contaminants in the envi-ronment. The aim of this work was to show the possibility of application of liquid chromatography in the study of the process of biodegradation is anticipated by activated sludge microorganisms. The content of Cefazolin in the solutions was controlled by the method of reversed-phase high-performance liquid chromatography (RP HPLC). During the first three hours of incubation is anticipated in the system with activated sludge has been a significant decrease is anticipated in the samples with concentrations of 10 and 40 mg/g of biomass. A marked decrease in the content of Cefazolin with a concentration of 70 mg/g of biomass was observed after four hours of incubation. Increase the incubation time was accompanied by a gradual decrease in the content of Cefazolin. Incubation for 24 hours resulted in almost complete removal of the antibiotic. Calculated average rate constant of biodegradation and the time half-life of the antibiotic.

 

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