I.A. Cherenkov - Ph.D. (Biol.), Associate Professor, Udmurt State University, Russia, Izhevsk T.N. Kropacheva - Ph. D. (Chem.), Associate Professor, Udmurt State University, Russia, Izhevsk E.A. Perevozchikov - Student, Udmurt State University, Russia, Izhevsk V.G. Sergeev - Dr. Sc. (Biol.), Professor, Udmurt State University, Russia, Izhevsk

In recent decades considerable attention is paid to bioelectrochemical systems (BESs) involving microbial cells which can be used as biosensors and fuel cell bioanodes. The relevance of this research lies in implementation of biotechnological po-tential of actinobacteria of the genus Rhodococcus as a component of BESs. The goal of this research is to model an electrochemical system based on Rhodococcus sp. cells with methylene blue as an electron transfer mediator. Rhodococcus sp. cells immobilization on the surface of the working graphite electrode was studied by luminescent and atomic force microscopy. This research proves efficiency of the combined cell immobilization which includes cells adsorption on the graphite electrode followed by coating with an agarose gel. Redox transformation of methylene blue on the electrode with immobilized cells was studied by cyclic voltammetry. It showed that the introduction of energy substrates was accompanied by the peak current increase corresponding to electrochemical oxidation processes of the methylene blue, whilst recovery current values remained almost unchanged. It was found that changes of the current intensity are depen-dent upon the concentration of an energy substrate.

 

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