A.I. Lebedeva - Ph.D. (Biol.), Senior Research Scientist, Morphology Department, FSBI «Russian Eye and Plastic Surgery Centre» of the Russian Federation Ministry of Health, Ufa city. Е-mail: Jeo102@mail.ru

The restoration of rough defects of the uterine smooth muscular tissue ends with the formation of the rough fibrous scar which results in the ineffectiveness of the reproductive function. The leveling of excessive fibrosis and stimulation of rege-nerative myometrium pool is a task of paramount importance for an adequate tissue regeneration. Alloplant biomaterial (ABM) is used to restore extensive tissue defects due to the induction of tissue elements. Material and methods. 25 female rabbits underwent CO2 laser beam incision in the uterine horn. In 10 animals (control) the defect wasclosedbyvicryl, whereasthedefects in 15 rabbits (experiment) was filled in by ABM. ABM is a transplant with volume fibrous structure, made for the given case from tendons of rabbits and treated as per Alloplant® technology devel-oped in the Federal State Budgetary Institution - The Russian Eye and Plastic Surgery Centre?, the city of Ufa. Histological, electron-microscopicandimmunohistochemical (PCNA) methodswere used for the investigation. Results. There were determined signs of the acute inflammation after inflicting a wound in the rabbit uterine horn wall during the early period (2-7 days). After 14 days there was observed a proliferative stage of the inflammation characteri-zedbythemacrophagalfibroblasticinfiltrationwith an activationofthebibroblasticfunction. There was no vigorous inflammatory reaction after the use of ABM. A massive migration of macrophages and the resorption by them of the biomaterial particles were determined. Macrophages induced by ABM biodegradation products through thesystemofmonokinespromoted-theregulation of cellular interactions with connective tissuecellsandleio¬myocytes. The proliferative phase of the inflammation (14 days) was taking place with the participation of the 1-st typecollagenblastswith a moderatecollagensyntheticfunction. Loose vascularized connective tissue was being formed in the place of the defect. Proliferativelyactivesmallsmooth myocytes and their low-differentiated precursors which transformed into mature formspenetratedintointerfibrillarspaces. Thus, ABM implantation into the wall of the rabbit uterine horn doesn-t cause a manifested inflammatory reaction. During the month it is subjected to resorption by macrophages and substituted by newly-formed loose connective tissue. The onsetoftheprolifetativephaseofinflammation is recorded after 14 days following the beginning of the experiment and is characterized by a weak fibroblastic activity, active proliferation of myocytes which result in the restoration of the myometrium of the rabbit uterine horn wall.

 

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