E.D. Sklyanchuk - Dr.Sc. (Med.), Professor, Head of Orthopedic Department, Center of Traumatology and Orthopedics of The Railway Clinical Hospital named Semashko N.A. at Lublin station of «Russian Railways», Moscow. E-mail: drevg@mail.ru A.A. Prosvirin - Assistant, Department of Traumatology and Orthopedics, Moscow State Medical and Dental University named A.I. Evdokimov. E-mail: homik24@mail.ru I.Yu. Malyshev - Dr.Sc. (Med.), Professor, Head of Department of Pathological Physiology, Moscow State Medical and Dental University named A.I. Evdokimov. E-mail: iymalyshev1@gmail.com I.S. Fadeeva - Ph.D. (Biol.), Research Scientist, Laboratory of Tissue Engineering, Institute of Teoretical and Experimental Biophysics of the Russian Academy of Sciences, Moscow region, Pushchino. E-mail: aurin.fad@gmail.com N.I. Fesenko - Ph.D. (Biol.), Associate Professor, Training Center of Biophysics and Biomedicine, Pushchino State Institute of Natural Science Ministry of Education and Science. E-mail: fesenko.natalia@rambler.ru V.N. Gorshenev - Ph.D. (Phis.-Math.), Senior Research Scientist, Institute of Biochemical Physics named N.M. Emanuel of the Russian Academy of Sciences, Moscow. E-mail: gor@sky.chph.ras.ru A.T. Teleshev - Dr.Sc. (Chim.), Professor of Chair of Physical and Analytical Chemistry, Moscow Pedagogical State University. E-mail: telechevat@rambler.ru Y.A. Ershov - Dr.Sc. (Chim.), Professor, Department of BMT2, Moscow State Technical University named of N.E. Bauman. E-mail: ershov_54@mail.ru V.V. Guriev - Dr.Sc. (Med.), Professor, Head of the Center of Traumatology and Orthopedics, Railway Clinical Hospital named N.A. Semashko at Lublin station of «Russian Railways». E-mail: drguriev@mail.ru

The results presented demonstrate the ability of macrophages to alter their phenotypic plasticity depending on the type of osteoplastic material, including the changes of their response to LPS activation. The results point to some mechanisms of inducing pro- and anti-inflammatory response in macrophages in response to exogenous osteoplastic materials. The effects of tested osteoplastic material on the M1 and M2 macrophages phenotypes characterized by moderate pro-inflammatory activation with a tendency to shift them towards an anti-inflammatory phenotype, acti-vating and regulating reparative processes. Development of inflammation in a wound in the presence of osteoplastic materials can be accompanied by enhanced both pro- and anti-inflammatory activity of macrophages.

 

1. Vanin A.F. Oksid azota v biomedicinskikh issledovanijakh // Vestnik RAMN. 2000. № 4. S. 3-5.
2. Ljamina S.V., Kruglov S.V., Kalish S.V., Malyshev I.JU.Reprogrammirovanie alveoljarnykh makrofagov - novaja vozmozhnost upravlenija immunnym otvetom. // Vestnik VolgGMU. 2011. Vyp. 4(40). S. 42-46.
3. Prosvirin A.A., Skljanchuk E.D., Gurev V.V. i dr. Fiziko-khimicheskie svojjstva i biosovmestimost nanostrukturirovannogo poristogo kostnogo implantata // Tekhnologii zhivykh sistem. 2013. T. 10. № 8. S. 68-73.
4. Somova L.M., Plekhova N.G. Oksid azota kak mediator vospalenija // Vestnik DVO RAN. 2006. № 2. S. 77-80.
5. Patent № 2482880 (RF).
6. SHvarc JA.SH., Svistelnik A.V. Funkcionalnye fenotipy makrofagov i koncepcija M1-M2 poljarizacii. CH.1 Provospalitelnyjj fenotip // Biokhimija. 2012. T. 77. S. 312-329.
7. Goldmann O., von Köckritz-Blickwede M., Höltje C. et al. Transcriptome Analysis of Murine Macrophages in Response to Infection with Streptococcus pyogenes Reveals an Unusual Activation Program // Infect Immun. 2007. V. 75. Issue 8. P. 4148-57.
8. Janeway C. A., Travers P., Walport M., Shlomchik M. The immune system in health and disease: Garland Science Publishing. 2005.
9. Klebanoff S.J. Myeloperoxidase: friend and foe // J. Leukocyte Biol. 2005. V. 77. № 1. R. 529-557.
10. Martinez F.O., Sica A., Mantovani A. et al. Macrophage activation and polarization // Front Biosci. 2008. V. 13. P. 453-461.
11. Tumitan A.R., Monnazzi L.G., Ghiraldi F.R. et al. c // Microbiol. Immunol. 2007. V. 51(10). P. 1021-1028.