G.V. Maksimov - Dr.Sc. (Biol.), Professor, Department of Biophysics, Faculty of Biology, Moscow State University, Moscow. E-mail: gmaksimov@mail.ru V.A. Trofimov - Dr.Sc. (Biol.), Professor, Head of the Department of Genetics, Biological Faculty, Mordovia State University. NP Ogareva Russia, Republic of Mordovia, Saransk. E-mail: geneticlab@yandex.ru D.I. Sidorov - Post-graduate Student, Department of Genetics, Junior Research Scientist, REC «Nanobiotechnology» Mordovia State University. NP Ogareva E-mail geneticlab@yandex.ru S.S. Kovalenko - Post-graduate Student, Department of Biophysics, Biological Faculty, Lomonosov Moscow State University, Moscow V.V. Shutova - Ph.D. (Biol.), Associate Professor, Faculty of Biology Biotechnology, Mordovia State University. NP Ogareva. E-mail vshutova@yandex.ru E.A. Goodilin - Corresponding Member, Professor, Russian Academy of Sciences, Department of Materials Science, Moscow State University

Using nanostructured substrates based on silver (NSP) obtained a significant enhancement of the Raman scattering (RS) of biological molecules (DNA, nucleotides, hemoglobin, carotenoids), which is important for the diagnosis of its content and conformation in the blood. In the analysis of red blood cells, using the NSP and recording RS is not from the cytoplasmic and from membrane bound hemoglobin localized at a distance of 10-15 nm from NSP. It is found that the RS hemoglobin of red blood cells in the NSP does not differ by location of the peaks, but more intense, especially in the low-frequency region spectrum. Found that, with the help of the RS and the NRS with greater sensitivity can be controlled by changing the concentration and molecular conformation carotene, registering both the magnitude of the RS intensity and the ratio of the value of the band intensities of the RS of carotene. This allows you to record the RS of carotene to plasma proteins, which is important for clinical diagnosis. It has been established, that reducing the amplitude of the functional groups of deoxyribose DNA RS indicates an increase rigidity \"framework\" of the double helix of DNA oligonucleotides at pathology. Change in the amplitude of the intensity RS of functional groups of nitrogenous bases, as well as the appearance of new bands indicates the structural changes within the DNA double helix, which may be due to local melting of DNA due to violation of the complementary mating modified nitrogenous bases, and perhaps there are gaps.

 

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