A.A. Lubyako - Dr.Sc.(Biol.), Professor, the Deputy of General director of Scientific work of Federal State Budgetary Institution of Research Center of Balneology and Rehabilitation of FMBA of Russia
G.Yu. Litvak - Doctor, the Head of office of laboratory of City clinical hospital No. 2 of Podolsk of the Moscow Region
V.K. Novikov - Dr.Sc.(Med.), Professor, Head of the Laboratory of Federal State Budgetary Institution Research Center of Balneology and Rehabilitation of FMBA of Russia
A.V. Barinov - Ph.D. (Med.), Associate Professor, Research Scientist, Federal State Budgetary Institution Research Center of Balneology and Rehabilitation of FMBA of Russia
A.S. Tyambina - Junior Research Scientist, Federal State Budgetary Institution Research Center of Balneology and Rehabilitation of FMBA of Russia
S.V. Svetlichnaja - Doctor, the Intern of Federal State Budgetary Institution Research Center of Balneology and Rehabilitation of FMBA of Russia
D.V. Bashlykov - Junior Research Scientist, Head of the laboratory of Federal State Budgetary Institution Research Center of Balneology and Rehabilitation of FMBA of Russia
A.V. Shipitsyn - Junior Research Scientist, Head of the laboratory of Federal State Budgetary Institution Research Center of Balneology and Rehabilitation of FMBA of Russia

In research on 194 patients with the diagnosis «SD-II» 210 transplantations of a cellular material of a xenogeny pancreas are executed. From them: to 18 patients are replaced β?cells of primary culture (3-6 million); to 30 primary culture of all Langer Hans cells (3-6 million), and 55 - a native suspension of cells of all Langer Hans cells (3-6-9 million) is replaced: 14 of them operation was executed repeatedly in 3,5 months after the first transplantation; to 30 patients 1-2 sessions of the artificial endocrine pancreas (AEP) were carried out. To 30 patients primary culture of all Langer Hans cells (3-6 million) was replaced during AEP session (1-2 sessions); to the 1 patient the native suspension of cells of all Langer Hans cells (9 million) was three times replaced every 3,5 month after the previous transplantation and in 1-2 weeks after AEP session (only 2 sessions), previous each transplantation. As group of comparison correction was made in stationary conditions insulin of short action. It is shown that in the general algorithm of complex treatment of sick SD, transplantation of primary culture or a native suspension of cells of all Langer Hans cells in combination with AEP sessions (according to indications) can be recommended to as an effective remedy of recovery correct treatment, regardless of terms of demonstration SD allowing in 80-84% cases to achieve steady good therapeutic effect.

 

1. Ancziferov M.B., Tokmanova A.Ju.. Galstyan G.R. i dr. Sindrom diabeticheskoj stopy'. Atlas. M. 2002.
2. Varshavskij I.M., Avdeeva T.V., Shabanov N.Ja. Diabeticheskaya stopa. Samara. 1999. S. 241.
3. Got'e S.V. Xirurgicheskie aspekty' transplantaczii abdominal'ny'x organov / In: «Ocherki klinicheskoj transplantologii». M. 2009. S. 27 - 56
4. Dedov I.I., Mel'nichenko G.A., Fadeev V.V. E'ndokrinologiya. M.: Mediczina. 2000. 631 s.
5. Dedov I.I., Udovichenko O.V., Galstyan G.R. Diabeticheskaya stopa. Moskva. 2005. 175 c.
6. Dibirov M.D., Kirtadze D.G., Tereshhenko S.N. i dr. Uluchshenie kachestva zhizni pozhily'x bol'ny'x s sindromom «Diabeticheskaya stopa» // Vestnik xirurgii. 2006. № 5. S. 89 - 93.
7. Dmitriev B.I., Torbinskij A.M., Kitajskij .S., Vansovich B.C. Metod lecheniya  diabeticheskoj stopy' // Gospital'naya xirurgiya. 2001. № 3. S. 33 - 35.
8. IvanovA.A. Perspektivy' primeneniyastvolovy'xkletokvmediczine / In: «Biologiyastvolovy'xkletokikletochny'etexnologii» // podred. akademikaM.A. Pal'czeva. M.: Mediczina. 2009. T. 1. S.31 - 43.
9. Kalinin A.P., Axunbaev M.I. Diabeticheskaya angiopatiya i gnojno-nekroticheskie oslozhneniya nizhnix konechnostej u bol'ny'x saxarny'm diabetom: Uchebno-metod. posobie. Bishkek. 1991. S. 82.
10. Litvak G.Ju., Barinov A.V., Komarov V.V., Tyunina G.K., Perova N.V., Sevast'yanov V.I., Lubyako A.A., Shumakov V.I. Transplantacziya pervichnoj kul'tury' ostrovkovoj tkani ksenogennoj podzheludochnoj zhelezy' bol'ny'm saxarny'm diabetom // Texnologii zhivy'x sistem. 2010. № 1. S. 19 - 27.
11. Litvak G.Ju. Diabeticheskaya stopa, profilaktika i kompleksnoe lechenie. M.: Izd-vo RUDN. 2009. 285 s.
12. Lubyako A.A., Kirpatovskij V.I., Slobin V.N. i dr. Texnologiya korrekczii narushenij funkczii organov, tkanej i ix sistem usovershenstvovanny'mi metodami organoterapii. M. 2006. 65 s.
13. Lubyako A.A., Ostapishin V.D., Tyambina A.S. Kletochnaya, tkanevaya i organnaya vosstanovitel'naya terapiya: Mediczinskaya texnologiya / pod red. professora V.V. Ujby'. M.: 2011. 77 s.
14. Novikov V.K. Korrekcziya vy'razhennoj giperglikemii posle kardioxirurgicheskix operaczij s ispol'zovaniem kompleksa iskusstvennoj podzheludochnoj zhelezy' // Sb. dokladov na nauchnoj konf. NII transplantologii i iskusstvenny'x organov. M. 2006
15. Onishhenko N.A., Zakir'yanov A.R., Klimenko E.D., Volodina A.V., Kobozeva L.P., Michunskaya A.B., Pozdnyakov O.M. Kletochnaya terapiya kak sposob realizaczii regeneraczionnoj strategii pri lechenii saxarnogo diabeta I tipa i ego oslozhnenij / In: «Biologicheskierezervy' kletokkostnogomozgaikorrekcziyaorganny'xdisfunkczij» / podred. V.I. Shumakova i N.A. Onishhenko. M.: Lavr. S. 127-152.
16. Onishhenko N.A., Krasheninnikov M.E. Kletochnaya transplantacziya ? perspektivnoe napravlenie regeneraczionnoj medicziny' / V kn. «Biologicheskie rezervy' kletok kostnogo mozga i korrekcziya organny'x disfunkczij» / pod red. V.I. Shumakova i N.A. Onishhenko. M.: Lavr. S. 49 - 76.
17. Romanov Ju.A., Smirnov V.N. Mezenximal'ny'e stvolovy'e kletki: biologiya i perspektivy' klinicheskogo primeneniya / In: «Biologiya stvolovy'x kletok i kletochny'e texnologii» / pod red. akademika M.A. Pal'czeva. M.: Mediczina. 2009. T. 1. S.193 - 205.
18. Svetuxin A.M., Zemlyanoj A.B. Gnojno-nekro­ticheskie formy' sindroma diabeticheskoj stopy' / Consilium medicum. 2002. T. 04. № 10. S. 537 - 544.
19. Sevast'yanov V.I., Lubyako A.A., Perova N.V. i dr. Pervy'j opy't primeneniya biodegradiruemogo matriksa Sfero®GEL' v vosstanovitel'noj xirurgii / Tezisy' dokladov na Mezhdunar. Kitajsko-Rossijskom simpoz. g. Astra­xan'. 19-22 sentyabrya 2007 g. S. 147 - 152.
20. Skaleczkij N.N., Goncharova T.N., Zasorina L.V. i dr. Ksenotransplantacziya kul'tur ostrovkovy'x kletok na puti dostizheniya dlitel'noj insulinozavisimosti u bol'ny'x saxarny'm diabetom I tipa. // Vestnik transplantologii i iskusstvenny'x organov. 2002. № 3. S. 85 - 86.
21. Timofeev A.V. Kletochno-populyaczionnaya organizacziya podzheludochnoj zhelezy' i primenenie kletochny'x texnologij v lechenii saxarnogo diabeta / V kn. «Biologiya stvolovy'x kletok i kletochny'e texnologii» / pod red. akademika M.A. Pal'czeva. M.: Mediczina. 2009. T. 2. S. 253 - 310.
22. Tolpekin V.E., Onishhenko N.A., Xubutiya A.Sh., Bogdanova N.B. Ksenoorgany' i tkani v transplantologii / In: «Transplantologiya (rukovodstvo)» / podred. akademikaV.I. Shumakova. M.: Mediczina. 1995. S. 93 - 106.
23. Frolov V.A., Drozdova G.A., Kazanskaya T.A., Bilibin D.P., Demurov E.A. Patologicheskaya fiziologiya. M.: 2002. 707 s.
24. Shumakov V.I., Blyumkin V.N., Skaleczkij N.N. i dr. Transplantacziya ostrovkovy'x kletok podzheludochnoj zhelezy' (ocherki). M.: Kanon. 1995. 381 s.
25. Brand P.W. The insensitive foot / In: Jahss M.H. ed. Disorders of the foot & ankle: medical and surgical management. 2d ed. Philadelphia: Saunders. 1991. R. 2173 - 2175.
26. Brink S.J. Insulinozavisimy'j saxarny'j diabet: diagnostika i lechenie / In: «Manual of endocrinology and Metabolism». Boston. New York-Toronto-London. 1994. P.777 ? 802.
27. Cavanagh P.R., Young M.J., Adams J.E. et al. Correlates of structure and function in the diabetic foot. / Diabetologia. 1991. V.134. Sup.2. R. 39.
28. Edelman S.V. Perspektivy' diagnostiki i lecheniya saxarnogo diabeta // In: «Manual of endocrinology and Metabolism». Boston. New York-Toronto-London. 1994. P.857 - 873.
29. Fass B. Diabeticheskij ketoaczidoz i giperosmolyarnaya koma / In: «Manual of endocrinology and Metabolism». Boston. New York-Toronto-London. 1994. P. 803 ? 824.
30. Griffin J.E., Ojeda S.R. Textbook of endocrine physiology / Fiziologiya e'ndokrinnoj sistemy'. M. 2008. 495 s. Oxford: University press. 2004. 495 p.
31. Lehninger A. Principles of Biochemistry. Worth Publishers. Inc, The Johns Hopkins University School of Medicine. 1985. P. 1056.
32. Lehninger A.L. Biochemistry. The molecular basis of cell structure and function / Worth Publishers. Inc. NY. 1972. 954 p. (p.323).
33. Meczler D.E. Bioximiya. M.: Mir. 1980. V.2. 606 p. (p.315).
34. Oyibo S.O., Jude E.B., Tarawneh I. et al. A comparison of two diabetic foot ulcer classification systems the Wagner and the University of Texas wound classification systems / Diabetes. Care. 2001. V. 24. № l. R. 84 - 88.
35. Salans L.D. Insulinozavisimy'j saxarny'j diabet: diagnostika i lechenie // In: «Manual of endocrinology and Metabolism». Boston. New York-Toronto-London. 1994. P.825 - 844.
36. Skyler J.S. Insulinozavisimy'j saxarny'j diabet: e'tiologiya, patogenez i princzipy' terapii / V kn. «Manual of endocrinology and Metabolism» / Boston. New York-Toronto-London. 1994. P. 759 - 776.
37. Wagner F.M. A classification and treatment program for diabetic neuropathic and dysvascular foot problems / In: The American Academy of Orthopedic Surgeons instructional course lectures. // St. Louis: Mosby Year Book. 1979. P. 143 ? 165.