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Journal Technologies of Living Systems №4 for 2014 г.
Article in number:
Influence of perinatal iron exposure on metabolic syndrome development in male Wistar rats
Keywords: iron metabolic syndrome insulin resistance dyslipoproteinemia
Authors:
A.A. Tinkov - Post-graduate Student, Department of Biochemistry, Orenburg State Medical Academy, Orenburg. E-mail: tinkov.a.a@gmail.com
E.V. Popova - Ph.D. (Med.), Associate Professor, Department of Biochemistry, Orenburg State Medical Academy, Orenburg. E-mail: pmvug@inbox.ru
A.A. Nikonorov - Dr.Sc. (Med.), Professor, Head of Department of Biochemistry, Orenburg State Medical Academy, Orenburg, E-mail: nikonorov_all@mail.ru
Abstract:
The primary aim of the current study was to estimate the effect of perinatal iron sulfate exposure on metabolic syndrome development. Perinatal iron exposure was modeled by feeding pregnant and lactating female rats with drinking water containing 3 mg/l iron sulfate (FeSO4-7H2O) (FePN). Intact rats obtained pure drinking water (Control). The male offspring was fed standard (STD) of high-fat diet (HFD) postnatally forming the following groups: Control-STD; FePN-STD; Control-HFD; FePN-HFD. It is estimated that perinatal iron exposure along with postnatal HFD-feeding resulted in a significant 23% increase in adipose tissue mass when compared to the HFD-controls. Animals in this group were also characterized by hyperglycaemia, hyperinsulinemia and significant increase in insulin resistance index HOMA-IR values in comparison to the HFD-control group. FePN-HFD rats had a 38% increased triglycerides level and significantly decreased apoprotein A1 to apoprotein B ratio when compared to the respective control group. Perinatal iron treatment significantly increased the level of triene conjugates in serum heptane phase in comparison to the control animals. The latter reflects activation of systemic oxidative stress. Elevated serum iron and ferritin levels if perinatal iron-treated group indicate cumulation of the metal in the rats - organism. While the majority of parameters studied were affected by the diet type, analysis of variance indicated the significant impact of perinatal iron exposure on animal morphometric parameters, insulin and triglyceride levels, apoprotein spectrum and serum iron and ferritin concentrations. Generally, the results indicate that perinatal iron exposure leads to cumulation of the latter in the organism, consequently leading to potentiation of diet-induced metabolic syndrome in rats. The possible mechanism of these changes is iron-induced activation of free-radical oxidation.
Pages: 10-19
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