350 rub
Journal Technologies of Living Systems №9 for 2013 г.
Article in number:
Influence of iron and copper salts on adipogenic effect of the high-fat diet and metal balance in the adipose tissue
Keywords: iron copper adipose tissue adipogenesis
Authors:
A.A. Tinkov - Post-graduate Student, Orenburg State Medical Academy. E-mail: tinkov.a.a@gmail.com
A.A. Nikonorov - Dr.Sc.(Med.), Professor, Orenburg State Medical Academy. E-mail: nikonorov_all@mail.ru
Abstract:
The aim of our study was to investigate the influence of combined and isolated action of iron and copper salts on morphometric parameters and metal concentration in adipose tissue of rats maintaining on standard and high-fat diet. Wistar rats obtained FeSO4-7H2O and CuSO¬4 separately and in combination with drinking water while being fed standard and high-fat diet. Control groups of animals obtained pure bottled water on standard or high-fat diet background. The most expressed increase in weight gain and adipose tissue content was observed in rats obtaining combination of metals and fed high fat diet in comparison to high-fat fed control group. At the same time mean adipocyte cross section square exceeded control values in metal-treated animals fed standard and high-fat fed diet. Analysis of hair metal content revealed dose dependence in Fe and Cu accumulation. Concentration of cadmium (Cd), nickel (Ni) and cobalt (Co) in rats - hair did not differ from the control values while arsenic level was lower than apparatus response limit. A mild decrease in chromium (Cr) and vanadium (V) content in hair was observed in experimental animals. Level of iron and copper in adipose tissue failed to correlate with hair concentration and incoming concentration of metals. Concentration of Fe in adipose tissue of rats seems to be dependent on metal treatment and the type of a diet. As such, high-fat diet elevated iron level in metal-treated rats. It is important to mention that high-fat diet consumption without metal treatment lead to a slight decrease in adipose tissue Fe content. Adipose tissue Cu level remained relatively stable. It is estimated that Fe and Cu consumption of high-fat and standard diet results in a marked decrease in adipose tissue Cr and V content. Both diet type and metal treatment had a significant impact on adipose tissue Cr and V concentration. Taking in account the insulinomimetic action of Cr and V it is possible that the decrease in their adipose tissue level can serve as one of pathogenic mechanisms leading to insulin resistance in obesity.
Pages: 55-64
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