I.V. Strekalovskiy, L.V. Shabasheva, T.A. Krylova, Y.V. Golubentseva, V.B. Popov

Experiments were carried out to investigate the role of eMSC transplantation in the correction of toxic lesions of tissues and organs in embryonic models: 1) extra-embryonic vasculogenesis defeat in rat embryos with subsequent remodeling, and 2) violation of hepatic hematopoiesis in the early fetal period in rats. In the first case rat embryos in early organogenesis period (9,3-10,5 GD) were cultured in a medium containing 5-fluorouracil, resulting in disrupted primary vascular network formation and the subsequent failure of yolk sac vessels branching. Exocoelomic injection of fluorescently labeled eMSC in on the day 2 of cultivation did not lead to the reorganization of the vasculature, but small clusters of cells were later detected along the damaged vessels. In the second model rats at 14 days of gestation were injected subcutaneously with 5-FU, resulting in marked signs of fetal anemia: drastic reduction in the number of red blood cells and increased amount of nuclear fetal erythroblasts in blood. Intraplacental injection of about 105 eMSC the evening of the same day resulted in normalization of the cellular composition of fetal blood, decreasing the number of yolk sac erythroblasts and emerging of secondary (liver) erythroid cells.