350 rub
Journal Technologies of Living Systems №8 for 2013 г.
Article in number:
Expirience of receiving transgene mice of SPF-category
Keywords: laboratory mice SPF-category embryonic stem cells chimeras animals-biomodels good manufacturing practice
Authors:
A.S. Chernov, A.A. Ovsepian, G.B. Telegin
Abstract:
Using the latest developments of experimental embryology, genetic engineering and microsurgical equipment it was succeeded to master and introduce in production of Nursery of «Pushchino» of FIBH Russian Academy of Sciences technology of receiving genetically modified mice a method of an injection of the embryonic stem cells (ESC) in a cavity blastocystes. This technology is known long ago around the world, but for Russia it is the first case when rigid standardization on selection of the conditions, used materials, reagents and checking, allowed to scale this technology for production of mice-biomodels of SPF category. Now the Nursery of FIBH RAS is the only production in Russia laboratory rodents of the SPF category, accredited AAALAC International since 2004. The control system of Nursery was estimated and certified as meeting the requirements ISO 9001:2008 for "Development of production (genetically modified animals), cultivation, the contents, delivery of small laboratory rodents for tests and researches".
Pages: 15-20
References

  1. Gondo Y. Trends in large-scale mouse mutagenesis: From genetics to functional genomic // Nat. Rev. Genet. 2008. V. 9. P. 803-810.
  2. Nicklas W., Kraft V., Meyer B. Contamination of transplantable tumors, cell lines, and monoclonal antibodies with rodent viruses // Lab.Anim.Sci. 1993. V. 43. P.296-299.
  3. Peterson N.C. From bench to cageside: Risk assessment for rodent pathogen contamination of cells and biologics // ILAR J. 2008. V.49. P.310-315.
  4. Mumphrey S.M., Changotra H., Moore T.N., Heimann-Nichols E.R., Wobus C.E., Reilly M.J., Moghadamfalahi M., Shukla D., Karst S.M. Murine norovirus 1 infection is associated with histopathological changes in immunocompetent hosts, but clinical disease is prevented by STAT1-dependent interferon responses // J.Virol. 2007. V.81. P.3251-3263.
  5. Moorehead R.A., Sanchez O.H., Baldwin R.M., Khokha R. Transgenic overexpression of IGF-II induces spontaneous lung tumors: A model for human lung adenocarcinoma // Oncogene. 2003. V. 22. P. 853-857.
  6. Dennis M.B. Humane endpoints for genetically engineered animal models // ILAR J. 2000. V.41. P.94-98.
  7. Conner D.A. Transgenic mouse colony management // Curr. Protoc. Mol. Biol. 2005. Suppl 71. P. 23.10.1-23.10.8.
  8. Robinson V, Anderson S, Carver J.A., Francis R.J., Hubrecht R., Jenkins E., Mathers K.E., Raymond R., Rosewell I., Wallace J., Wells D.J. Refinement and reduction in production of genetically modified mice // Sixth report of the BVAAWF/FRAME/RSPCA/UFAW Joint Working Group on Refinement. Lab.Anim. 2003. V. 37 (Suppl 1). P.S1-S51.
  9. Conner D.A. Mouse colony management // Curr. Protoc. Mol. Biol. 2002. Suppl 57. P.23.8.1-23.8.11.
  10. Liu L, Nutter LMJ, Law N, McKerlie C. Sperm freezing and in vitro fertilization on three substrains of C57BL/6 mice // JAALAS. 2009. V.48. P. 39-43.