N.V. Besruchko, G.K. Rubsov

The study of oxidative modification of proteins (OMP) can be performed based on the reaction with 2,4-dinitrophenylhydrazine, is a reagent for carbonyl compounds. The base model of the biological system (MBS) for relevant research can serve yolk lipoproteins in a spontaneous and Fe2+-induced oxidation. Another series of MBS were the bee products (propolis, homogenate drone brood, honey, royal jelly) as a test system, with antioxidant properties. MBS considered adding serum rats as biological environment, sensitive to changes in the conditions of free-radical processes. Purpose - to justify the biological model system to test the method for assessing spontaneous and initiated by the oxidative modification of proteins in a pool of molecules of average weight. Justification MBS to test method for assessing spontaneous and initiated by OMP in a pool of MSM conducted in the following series of observations: 1) in the MBS with the addition of yolk lipoprotein and without the addition of yolk lipoproteins in the spontaneous and Fe2+-induced oxidation, and 2) the IBS with and without the addition of serum the blood of rats with spontaneous and Fe2+-induced oxidation, and 3) the IBS with and without the addition of yolk lipoprotein and serum of rats with spontaneous and Fe2+-induced oxidation. The biochemical characteristics of possible MBS through a comparative evaluation in terms of spontaneous and initiated by OMP in a pool of MSM to support the possible use of their complex. Is set at the analyzed model of biological systems to the conditions of Fe2+-induced oxidation, which showed changes in levels of analyzed tests, compared to spontaneous oxidation.

 

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