350 rub
Journal Technologies of Living Systems №9 for 2012 г.
Article in number:
The role of Snail signaling pathway in the development of resistance to hypoxia in breast cancer cells
Keywords: epithelial-mesenchymal transition breast cancer Snail1 ER&#945 estrogens hypoxia
Authors:
A.M. Scherbakov, L.B. Stefanova, O.E. Andreeva, D.V. Sorokin, M.A. Krasilnikov
Abstract:
The main goal of the project was to study a relationship between Snail signaling pathway, the hormonal response and the tolerance of breast cancer cells to hypoxia. Three in vitro cultured breast cancer cell lines were used: the hormone dependent MCF-7 cells and the hormone resistant ER-negative HBL-100 and MDA-MB-231 cells. We have established a direct relation between one of epithelial-mesenchymal proteins, Snail1 and the tolerance of breast cancer cells to hypoxia; we have demonstrated the participation of Snail1 in the cell survival under hypoxic conditions. The ER-dependent negative regulation of Snail1 and the activation of Snail1 in the hormone resistant breast cancer cells have been observed. In general, the results demonstrate the protective role of Snail1 in breast cancer, and show that Snail1 may serve as perspective target in the treatment of ER-negative breast cancer.
Pages: 63-67
References
  1. Берштейн Л.М., Сантен Р. Молекулярные механизмы резистентности к тамоксифену // Вопросы онкологии. 2002. V. 48. P. 17 - 23.
  2. Красильников М.А. Современные подходы к изучению механизма эстроген-независимого роста опухолей молочной железы // Вопросы онкологии. 2004. V. 50. P. 399 - 405.
  3. Normanno N., Di Maio M., De Maio E., De Luca A., de Matteis A., Giordano A., Perrone F. Mecha­nisms of endocrine resistance and novel therapeutic strategies in breast cancer // Endocr Relat Cancer. 2005. V. 12. P. 721 - 747.
  4. Chang J., Fan W. Endocrine Therapy Resistance: Current Status, Possible Mechanisms and Overcoming Strategies // Anticancer Agents Med Chem. 2012, epub.
  5. van Agthoven T., Sieuwerts A.M., Meijer-van Gelder M.E., Look M.P., Smid M., Veldscholte J., Sleijfer S., Foekens J.A., Dorssers L.C. Relevance of breast cancer antiestrogen resistance genes in human breast cancer progression and tamoxifen resistance // Journal of clinical oncology: official journal of the American Society of Clinical Oncology. 2009. V. 27. P. 542 - 549.
  6. Musgrove E.A., Sutherland R.L.Biological deter­minants of endocrine resistance in breast cancer // Nat Rev Cancer. 2009. V. 9. P. 631 - 643.
  7. Arpino G., De Angelis C., Giuliano M., Giordano A., Falato C., De Laurentiis M., De Placido S. Molecular mechanism and clinical implications of endocrine therapy resistance in breast cancer // Oncology. 2009. V. 77. Suppl 1. P. 23 - 37.
  8. Scherbakov A.M., Lobanova Y.S., Shatskaya V.A., Onopchenko O.V., Gershtein E.S., Krasil'nikov M.A. Activation of mitogenic pathways and sensitization to estrogen-induced apoptosis: two independent characteristics of tamoxifen-resistant breast cancer cells - // Breast Cancer Res Treat. 2006. V. 100. P. 1 - 11.
  9. Zhou J., Schmid T., Schnitzer S., Brune B. Tumor hypoxia and cancer progression // Cancer letters. 2006. V. 237. P. 10 - 21.
  10. Brahimi-Horn M.C., Pouyssegur J.The hypoxia-inducible factor and tumor progression along the angiogenic pathway // Int Rev Cytol. 2005.  V. 242. P. 157 - 213.
  11. Schito L., Rey S., Tafani M., Zhang H., Wong C.C., Russo A., Russo M.A., Semenza G.L. Hypoxia-inducible factor 1-dependent expression of platelet-derived growth factor B promotes lymphatic metastasis of hypoxic breast cancer cells // Proceedings of the National Academy of Sciences of the United States of America. 2012. V.109(40). P. 2707 - 2716.
  12. Scherbakov A.M., Andreeva O.E., Shatskaya V.A., Krasil'nikov M.A. The relationships between snail1 and estrogen receptor signaling in breast cancer cells // Journal of cellular biochemistry. 2012. V. 113. P. 2147 - 2155.
  13. Krasil'nikov M.A., Shatskaya V.A., Stavrov­skaya A.A., Erohina M., Gershtein E.S., Adler V.V. The role of phosphatidylinositol 3-kinase in the
    regulation of cell response to steroid hormones // Biochim Biophys Acta. 1999. V. 1450. P. 434 - 443.
  14. Vincent T., Neve E.P., Johnson J.R., Kukalev A., Rojo F., Albanell J., Pietras K., Virtanen I., Philipson L., Leopold P.L., Crystal R.G., de Herreros A.G., Moustakas A., Pettersson R.F., Fuxe J. A SNAIL1-SMAD3/4 transcriptional repressor complex promotes TGF-beta mediated epithelial-mesenchymal transition // Nat Cell Biol. 2009. V. 11.  P. 943 - 950.
  15. Lobanova Y.S., Scherbakov A.M., Shatskaya V.A., Evteev V.A., Krasil'nikov M.A.NF-kappaB suppression provokes the sensitization of hormone-resistant breast cancer cells to estrogen apoptosis // Molecular and cellular biochemistry. 2009. V. 324. P. 65 - 71.