I.A. Talashova

A technology for releasing calcium phosphate compounds (CPC) from the bone tissue of agricultural animals has been developed at Russian Ilizarov State Budgetary Institution RISC "RTO" of the RF Ministry of Healthcare and Social Development (Kurgan, Russia). Biodegradable composite implantation materials similar to bone in composition have been produced on their basis. When these materials are used, bone defect filling with newly formed bone tissue occurs after 1-2 months. Aim of the study was to determine the effect of the implantation materials developed on the metabolic processes of experimental animals, as well as to evaluate their biocompatibility. Method. The experiment on implantation of the materials developed was performed using 36 adult mongrel dogs, both males and females, at the age from one to three years the body weight of which amounted to 8.8±3.2 kg. Cone-shaped non-through defects (perforations) of 5-mm diameter and 7-mm of height were produced in the animals - humeral and tibial metaphyses in sterile conditions under general anesthesia using a drill. The defects were filled with the composite materials studied. Biochemical testing of blood serum was performed before surgery and every week up to 6 weeks thereafter using HITACHI 902 (USA) automatic biochemical analyzer and reagents by «Vital Diagnostics» (Russia). General protein (GP), the activity of general alkaline phosphatase (GAP) and tartrate-resistant acid phosphatase isoenzyme (TRAP) was determined, as well as Ca, Mg, Cl and P concentrations. The content of serum protein fractions was determined by electrophoresis technique using the equipment and reagent sets by "Beckman-Coulter" (USA). Results. The values of the parameters studied suggest that implantation of the materials developed has no significant effect on canine metabolic processes. The parameter values in all the experimental groups didn-t differ from preoperative ones by the end of experiment. It has been established that making the organic substances derived from xeno-tissues a component of composite implantation materials leads to more marked changes in metabolic processes of experimental animals comparing with the cases when the substances derived from the tissues of same-species animals are used as organic components.