350 rub
Journal Technologies of Living Systems №7 for 2010 г.
Article in number:
VASOPROTECTIVE ACTION OF SUPEROXIDE DISMUTASE-CHONDROITIN SULFATE-CATALASE CONJUGATE IN PREVENTIVE AND ACUTE APPLICATION ON HEMODYNAMIC CHANGES INDUCED WITH HYDROGEN PEROXIDE
Keywords: blood pressure heart rate oxidative stress hydrogen peroxide bienzyme superoxide dismutase-chondroitin sulfate-catalase conjugate antioxidant therapy
Authors:
A.V. Vavaev, V.L. Lakomkin, E.G. Tischenko, V.I. Kapelko, A.V. Maksimenko
Abstract:
The influence of superoxide dismutase-chondroitin sulfate-catalase (SOD-CHS-CAT) conjugate was studied in respect to hemodynamics induces and electrocardiogram (ECG) of rabbit and rats at normal and oxidative stress conditions. The oxidative stress was induced by intravenous injection of hydrogen peroxide. The bienzyme SOD-CHS-CAT conjugate is safe for application in vivo and possesses the pronounced normalizing action on hemodynamics indices after its intravenous bolus administration. The ECG has not significant alteration. For the first time it was found the prophylaxy action of SOD-CHS-CAT conjugate in vivo against sprout oxidative stress. The further investigation and development of oral forms of bienzyme SOD-CHS-CAT conjugate for prophylaxy application is going to come the important stage for progress of antioxidant therapy.
Pages: 15-21
References
  1. Maksimenko A.V., Tischenko E.G. Macromolecular ensembles of internal and external fibrinolysis: the resources for enhancement of thrombolysis efficacy // Curr. Med. Chem. 2006. V. 13. N. 14. P. 1617-1625.
  2. Taylor J.K., Gardner T.B., Waljee A.K., Dimagno M.J., Schoenfeld P.S. Systematic review: efficacy and safety of pancreatic enzyme supplements for exocrine pancreatic insufficiency // Aliment Pharmacol. Ther. 2010. V. 31. N. 1. P. 57-72.
  3. Maksimenko A.V.Experimental antioxidant biotherapy for protection of the vascular wall by modified forms of superoxide dismutase and catalase // Curr. Pharm. Design. 2005. V. 11. N. 11. P. 2007-2016.
  4. Максименко А.В. Ваваев А.В.Ферментные антиоксиданты - следующий этап фармакологического противостояния окислительному стрессу - // Молекулярная медицина. 2010.
    № 2. С. 9 - 14.
  5. Максименко А.В. Внеклеточное оксидативное поражение сосудистой стенки и её ферментная антиоксидантная защита // Хим.-фарм. журн. 2007. Т. 41. № 5. С. 3 - 12.
  6. Nakajima H., Homgaishi M., Ishizaka N., Taguchi J., Igarashi R., Mizushima Y., Nagai R., Ohno M. Lecithinized copper zinc-superoxide dismutase ameliorates ischemia-induced myocardial damage // Life Sci. 2001. V. 69. N. 8. P. 935-944.
  7. Ishigara T., Tanaka K., Tasaka Y., Namba T., Suzuki J., Ishihara T., Okamoto S., Hibi T., Takenaga M., Tgarashi R., Sato K., Mizushima Y., Mizushima T. Therapeutic effect of lecithinized superoxide dismutase against colitis // J. Pharmacol. Exp. Ther. 2009. V. 328. № 1. P. 152-164.
  8. Suzuki Y. Matsumoto T., Okamoto S., Hibi T. A lecithinized superoxide dismutase (PC-SOD) improves ulcerative colitis // Colorectal. Dis. 2008. V. 10. N. 9. P. 931-934.
  9. Maksimenko A.V., Golubykh V.L., Tischenko E.G. The combination of modified antioxidant enzymes for anti-thrombotic protection of the vascular wall: the significance of covalent connection of superoxide dismutase and catalase activities // J. Pharmacy. Pharmacol. 2004.  V. 56.
    N. 11. P. 1463-1468.
  10. Максименко А.В., Тищенко Е.Г. Модификация каталазы хондроитинсульфатом // Биохимия. 1997. Т. 62. № 10. С. 1364-1368.

  12. Капелько В.И.Регуляторная роль кислородных радикалов в миокардиальных клетках // Рос. физиол. журнал. 2004. Т. 90. № 6.
    С. 681-691.
  13. Tanaka K.I., Ishihara T., Azuma A., Kudoh S., Ebina M., Nukiwa T., Sugiyama Y., Tasaka Y., Namba T., Ishihara T., Sato K., Mizushima Y., Mizushima T. Therapeutic effect of lecithinized superoxide dismutase on bleomycin-induced pulmonary fibrosis// Am. J. Physiol. Lung. Cell. Mol. Physiol. 2010. V. 298. N. 3. P. L348-L360.
  14. Lass A., Agarwal S., Sohal R.S. Mitochondrial ubiquinone homologues, superoxide radical generation, and longevity in different mammalian species // J. Biol. Chem. 1997. V. 272. N. 31.
    P. 19199-19204.
  15. Maksimenko A.V. Vavaev A.V., Tischenko E.G. Extracellular protection of vascular wall with the covalently connected antioxidant enzymes against oxidative damage. Abstract of the Thirteenth International Biotechnology Symposium and Exhibition, October 12-17, 2008, Dalian, China // J. Biotechnol. 2008. V. 136/S. P. S87.
  16. Prasa D., Stone G.W., Holmes D.R., Gersh B.
    Reperfusion injury, microvascular dysfunction, and cardioprotection: the «dark side» of reperfusion // Circulation. 2009. V. 120. N. 21.
    P. 2105-2112.