350 rub
Journal Technologies of Living Systems №3 for 2010 г.
Article in number:
ESTIMATION OF EFFICIENCY OF AUTOMATED NUCLEIC ACID EXTRACTION SYSTEMS
Keywords: automatic nucleic acid extraction systems sorption pneumovacuum method magnetic rotation method
Authors:
O.S. Antonova, N.A. Korneva, A.M. Tsygankov, Y.V. Belov, D.A. Varlamov, Y.V. Kyurdzhiev, A.V. Chernishev
Abstract:
The article is devoted to the analysis of work of two automatic systems of the test preparation, realizing the serial processing of the initial material by various agents, as well as washing, sedi-mentation and hashing of the sorption carrier, etc. The results were compared to the data received during the process of manual extraction of DNA by a method, used in devices. The fact is that during working with both devices the DNA output has exceeded the values received during the manual extraction by means of the same protocol. There is no basic difference at the estimation of DNA output revealed, extracted by means of open and air-tight systems, that allows us to use leaky system on the basis of the magnetic rotation method for daily medical diagnostics, and to use the device on the basis of pneumovacuum system for work with dangerous microorganisms.
Pages: 47-52
References
  1. Алексеев Я.И., Белов Ю.В., Варламов Д.А., Коновалов С.В., Курочкин В.Е., Маракушин Н.Ф., Петров А.И., Петряков А.О., Румянцев Д.А., Скоблилов Е.Ю., Соколов В.Н., Фесенко В.А., Чернышев А.В. Приборы для диагностики биологических объектов на основе метода полимеразной цепной реакции в реальном времени (ПЦР-РВ) // Научное приборостроение. 2006. Т. 16. № 3. С. 132-136.
  2. Hughes C., Galea-Lauri J., Farzaneh F., Darling D. Streptavidin paramagnetic particles provide a choice of three affinity-based capture and magnetic concentration strategies for retroviral vectors // Mol. Ther. 2001. V. 3. № 4. Р. 623-630.
  3. Dubus S., Gravel J.F., Le Drogoff B., Nobert P., Veres T., Boudreau D. PCR-free DNA detection
    using a magnetic bead-supported polymeric transducer and microelectromagnetic traps // Anal. Chem. 2006. V. 78. №13. Р. 4457-4464.
  4. Deggerdal A., Larsen F.Rapid isolation of PCR-ready DNA from blood, bone marrow and cultured cells, based on paramagnetic beads // Biotechniques. 1997. V. 22. №3. Р. 554-557.
  5. Verhofstede C., Fransen K., Marissens D., Verhelst R., van der Groen G., Lauwers S., Zissis G., Plum J. Isolation of HIV-1 RNA from plasma: evaluation of eight different extraction methods // J. Virol. Methods. 1996. V. 60. № 2. Р. 155-159.
  6. Berensmeier S.Magnetic particles for the separation and purification of nucleic acids // Appl Microbiol. Biotechnol. 2006. V. 73.P. 495-504.