I.A. Kirilova, V.T. Podorozhnaya, M.A. Sadovoy, V.A. Yudytskiy, N.G.Fomichev, L.M. Gordeeva

Microbial contamination of donor bone fragments was studied after non-sterile procurement and before sterilization. The first stage of the study included sterility control performed by bacteriolog-ical methods. Isolated culture was inoculated into selective nutrient media using culture tech-niques at the second stage. The third stage included identification of microorganisms up to species using biochemical methods. The study results showed that microbial landscape was presented by gram-positive microflora and yeast cultures. Pathogenic gram-negative microflora was not identified. Medical products prepared from such bone fragments are pyrogen-free.