350 rub
Journal Technologies of Living Systems №2 for 2009 г.
Article in number:
Nfatc4 gene polymorphism and aerobic performance in athletes
Keywords: NFATC4 gene polymorphism aerobic performance
Authors:
I.I. Ahmetov, D.V. Popov, Yu.V. Shikhova, S.S. Missina, O.A. Sarayev, O.L. Vinogradova, V.A. Rogozkin
Abstract:
Nuclear factor of activated T cells C4 gene (NFATC4) encodes transcription factor which regulates cardiac and skeletal muscle metabolism. The aim of the study was to investigate allelic distribution of NFATC4 gene Gly160Ala polymorphism in endurance-oriented athletes (n = 549) and controls (n = 1057), and to find interrelation between genotypes and physiological parameters in rowers (n = 90). Genotyping was performed by restriction fragment length polymorphism analysis. Physiological parameters were evaluated by PM 3 Rower Ergometer and MetaMax 3B Gas Analyzer. The frequency of NFATC4 Gly allele was significantly higher in athletes than in controls (51.5% vs. 43.7%; p < 0.0001), and increased with the growth of skills (sub-elite athletes: 36.7%?48.5%; highly elite athletes: 60.6%?62.5%). Furthermore, NFATC4 Gly allele was associated with high values of aerobic performance (when VO2max and AT in % of VO2max were measured). Thus, NFATC4 gene Gly160Ala polymorphism is associated with aerobic performance of athletes and plays an important role in sports selection
Pages: 24-30
References
  1. Ахметов И.И., Астратенкова И.В., Рогозкин В.А. Ассоциация полиморфизма гена PPARD с физической деятельностью человека // Молекулярная биология. 2007. Т.41. № 5.  С. 852-857.
  2. Allen D.L., Sartorius C.A., Sycuro L.K., Leinwand L.A. Different Pathways Regulate Expression of the Skeletal Myosin Heavy Chain // Genes. 2001. V. 276(47). P. 43524-43533.
  3. Benedito A.B., Lehtinen M., Massol R., Lopes U.G., Kirchhausen T., Rao A., Bonni A. The Transcription Factor NFAT3 Mediates Neuronal Survival // J. Biol. Chem. 2005. V. 280(4).  P. 2818-2825.
  4. Bolla M.K., Haddad L., Humphries S.E., Winder A.F., Day I.N.M. A method of determination of hundreds of APOE genotypes utilizing highly simplified, optimized protocols and restriction digestion analysis by microtitre array diagonal gel electrophoresis (MADGE) // Clin. Chem. 1995. V. 41. P. 1599-1604.
  5. Bushdid P.B., Osinska H., Waclaw R.R., Molkentin J.D., Yutzey K.E. NFATc3 and NFATc4 Are Required for Cardiac Development and Mitochondrial Function // Circ. Res. 2003. V. 92.  P. 1305-1313.
  6. Chin E.R., Olson E.N., Richardson J.A., Yang Q., Humphries C., Shelton J.M., Wu H., Zhu W., Bassel-Duby R., Williams R.S. A calcineurin-dependent pathway controls skeletal muscle fiber type // Genes Dev. 1998. V.12. P. 2499-2509.
  7. Crabtree G.R. Calcium, calcineurin, and the control of transcription // J. Biol. Chem. 2001.  V. 276. P. 2313-2316.
  8. Graef I.A., Chen F., Crabtree G.R. NFAT signaling in vertebrate development // Curr. Opin. Genet Dev. 2001. V.11. P. 505-512.
  9. Hagerman F.C. Applied physiology of rowing // Sports Med. 1984. V.1(4). P. 303-26.
  10. Hitomi Y., Kizaki T., Katsumura T., Mizuno M., Itoh C.E., Esaki K., Fujioka Y., Takemasa T., Haga S., Ohno H. Effect of moderate acute exercise on expression of mRNA involved in the calcineurin signaling pathway in human skeletal muscle // IUBMB Life. 2003. V. 55(7). P. 409-13.
  11. Ho I.C., Kim J.H., Rooney J.W., Spiegelman B.M., Glimcher L.H. A potential role for the nuclear factor of activated T cells family of transcriptional regulatory proteins in adipogenesis // Proc. Natl. Acad. Sci. USA. 1998. V. 95. P. 15537-15541.
  12. Hoey T., Sun Y.L., Williamson K., Xu X. Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins // Immunity. 1995. V. 2. P.461-472.
  13. Hogan P.G., Chen L., Nardone J., Rao A. Transcriptional regulation by calcium, calcineurin, and NFAT // Genes Dev. 2003. V. 17.  P. 2205-2232.
  14. Molkentin J.D., Lu J.R., Antos C.L., Markham B., Richardson J., Robbins J., Grant S.R., Olson E.N. A calcineurin-dependent transcriptional pathway for cardiac hypertrophy // Cell. 1998. V.93. P. 215-228.
  15. Molkentin JD. Calcineurin and beyond: cardiac hypertrophic signaling // Circ. Res. 2000. V.87. P. 731-738.
  16. Moore ML, Wang GL, Belaguli NS, Schwartz RJ, McMillin JB. GATA-4 and serum response factor regulate transcription of the muscle-specific carnitine palmitoyltransferase I beta in rat heart // J. Biol. Chem. 2001. V. 276. P. 1026-1033.
  17. O'Connor R.S., Mills S.T., Jones K.A., Ho S.N., Pavlath G.K. A combinatorial role for NFAT5 in both myoblast migration and differentiation during skeletal muscle myogenesis // J. Cell. Sci. 2007. V.120(Pt. 1). P. 149-59.
  18. Poirier O., Nicaud V., McDonagh T., Dargie H.J., Desnos M., Dorent R., Roizès G., Schwartz K., Tiret L., Komajda M., Cambien F. Polymorphisms of genes of the cardiac calcineurin pathway and cardiac hypertrophy // Eur. J. Hum. Genet. 2003. V.11(9). P. 659-64.
  19. Shikhova J.V., Ahmetov I.I., Lyubaeva E.V., Astratenkova I.V., Vinogradova O.L., Rogozkin V.A. NFATC4 gene variation is associated with muscle fiber composition of athletes // Eur. J. Hum. Genet. 2007. V. 15 (Supp. 1). P. 264-265.
  20. Tomita M., Reinhold M.I., Molkentin J.D., Naski M.C. Calcineurin and NFAT4 Induce Chondrogenesis // J. Biol. Chem. 2002.  V. 277(44). P. 42214-42218.
  21. Xia Y, McMillin JB, Lewis A, Moore M, Zhu WG, Williams RS, Kellems RE. Electrical stimulation of neonatal cardiac myocytes activates the NFAT3 and GATA4 pathways and up-regulates the adenylsuccinate synthetase 1 gene // J.  Biol. Chem. 2000. V. 275. P. 1855-1863.
  22. Yang T.T.C., Suk H.Y., Yang X.Y., Olabisi O., Yu R.Y.L., Durand J., Jelicks L.A., Kim J-Y., Scherer P.E., Wang Y., Feng Y., Rossetti L., Graef I.A., Crabtree G.R., Chow C-W. Role of Transcription Factor NFAT in Glucose and Insulin Homeostasis // Mol. Cell. Biol. 2006.  P. 7372-7387.
  23. Yang T.T.C., Xiong Q., Enslen H., Davis R.J., Chow C-W. Phosphorylation of NFATc4 by p38 Mitogen-Activated Protein Kinases // Mol. Cell. Biol. 2002. V. 22(11). P. 3892-3904.
  24. Zaichuk T.A., Shroff E.H., Emmanuel R., Filleur S., Nelius T., Volpert O.V. Nuclear factor of activated T cells balances angiogenesis activation and inhibition // J. Exp. Med. 2004. V. 199. P. 1513-1522.