V.V. Sabelnikov - Ph. D. (Eng.), Associate Professor, Bauman Moscow State Technical University E-mail: smitr@mail.ru T.M. Sabelnikova - Ph. D. (Eng.), Associate Professor, Bauman Moscow State Technical University E-mail: smitr@mail.ru V.N. Goryacheva - Ph. D. (Chem.), Associate Professor, Bauman Moscow State Technical University E-mail: vanigor2009@jandex.ru

The paper considers the experimental results directed to increase the bactericidal potential of low-frequency ultrasound due to sonication of bacterial suspensions under the conditions of the external excessive static pressure. Experiments on the most common samples of the infected wound microflora, such as Staphilococcus aureus, Proteus vulgaris, Escherichia coli and Pseudomonas aeruginosa were conducted at Bauman Moscow State Technical University and in Burdenko Chief Military Clinical Hospital. Tests were carried out in vitro while sonicating 50 ml of each tested bactericidal suspension with the concentration of (40-45)۰103 microbial bodies per one milliliter of solution, which, as a rule, corresponds to the microbial landscape of a typical infected wound. Serial ultrasound equipment of URSC-7N model was used during the experiments. It provided the following oscillation parameters: operating frequency f = 26.5 kHz, concentrate and shifting amplitude A = 30-50 µm. The maximal bactericidal effect is achieved by imposing the external excessive static pressure on the sonicated microflora within 0.4-0.7 excess pressure limits. In this case, the time of complete sterilization of bacterial suspensions is 2.5-2.7 times shorter than the time of complete suppression of the pathogenic microflora during the common sonication process. The results of the experiments caused the development of a new approach for ultrasound treatment of infected wounds at Bauman Moscow State Technical University.

 

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