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Journal Science Intensive Technologies №11 for 2016 г.
Article in number:
Effect of sonicated solution temperature on bactericidal ability of low-frequency ultrasound
Keywords: low-frequency ultrasound wound infection bacterial flora bactericidal effect of ultrasound cavitation temperature range antiseptics of weak concentration time of complete sterilization
Authors:
T.M. Sabelnikova - Ph. D. (Eng.), Associate Professor, Bauman Moscow State Technical University E-mail: smitr@mail.ru V.V. Sabelnikov - Ph. D. (Eng.), Associate Professor, Bauman Moscow State Technical University E-mail: smitr@mail.ru V.N. Goryacheva - Ph. D. (Chem.), Associate Professor, Bauman Moscow State Technical University E-mail: vanigor2009@jandex.ru
Abstract:
The results of the studies directed to increase bactericidal potential of low-frequency ultrasound due to optimal heating of sonicated bacterial solutions are provided in the article. Experiments in the most common representatives of the infected wound microflora: Staphylococcus aureus, proteus, Escherichia coli and Pseudomonas aeruginosa were conducted in Bauman Moscow State Technical University and Burdenko Chief Military Clinical Hospital. Tests were performed in vitro while sonicating of 50 ml of each tested bactericidal suspension with concentration of 40-45∙103 microbial bodies per one milliliter of solution which as a rule corresponds to the microbial landscape of the typical infected wound. Serial ultrasound apparatus of URSC-7N model provided the following oscillation parameters: working frequency f = 26.5 kHz and concentrate end shifting amplitude A = 30-50 µm was used in experiments. Maximal increase of cavitational processes is noted heating of solutions within a relatively narrow temperature range: 37-42°C for Staphylococcus aureus, proteus, Escherichia coli and 25-30°C for Pseudomonas aeruginosa that in our opinion increases the ultrasound degenerative effect on sonicated microorganisms. In this case the time of complete sterilization of bacterial suspensions decreases by 25-30% in relation to the time of complete suppression of the pathogenic microflora at the common sonication process. Experimentally established optimal temperatures of bacterial suspension heating mainly meet to temperature ranges at which most favorable conditions are created for growth of all tested microorganism species. The results of the performed studies were used in the new method of ultrasound treatment for infected wounds developed in Bauman Moscow State Technical University and passed successful testing in Burdenko Chief Military Clinical Hospital.
Pages: 38-43
References

 

  1. Kuzin M.I., Kostjuchenok B.M. Rany i ranevaja infekcija. M.: Medicina. 1999.
  2. Frolova A.V., Kosinec A.N., Okulich V.K. Ranevaja infekcija. Sostojanie problemy //       Vestnik Vitebskogo Gosudarstvennogo medicinskogo universiteta. 2014. T. 13. № 2. S. 62−63.
  3. Akopjan B.V., Ershov JU.A. Osnovy vzaimodejjstvija ultrazvuka s biologicheskimi obektami: Ucheb. posobie. M: Izd-vo MGTU im. N.EH. Baumana. 2005. 224 s.
  4. Kiselev M.T., Minchenja V.G., Stepanenko D.A. Ultrazvuk v medicine: monografija. Minsk: BNTU. 2009. 427 s.
  5. Physical Principles of Medical Ultrasonics. Ultrazvuk v medicine. Fizicheskie osnovy primenenija: Per. s angl. / Pod. red. K. KHilla. M.: Fizmatlit. 2008. 542 s.
  6. Alkov S.V., Gadzhiev I.S., Petrov V.I. Klinicheskie nabljudenija primenenija ultrazvukovojj obrabotki biologicheskikh tkanejj // Biomedicinskaja radioehlektronika. 2013. № 1. S. 83−85.
  7. Patent RF № 20882467 ot 27.06.1997. Sposob ultrazvukovojj obrabotki inficirovannykh ran i ustrojjstvo dlja ego osushhestvlenija / Sabelnikov V.V., Loshhilov V.I., Sabelnikova T.M.
  8. Sabelnikova T.M., Sabelnikov V.V. Razrabotka i sozdanie ustrojjstva dlja ultrazvukovojj obrabotki inficirovannykh ran // Inzhenernyjj vestnik. EHlektronnyjj nauchno-tekhnich. zhurnal MGTU im. N.EH. Baumana. Dekabr 2014. № 12. S. 600−606.
  9. Sabelnikova T.M., Sabelnikov V.V. Metod ultrazvukovojj obrabotki inficirovannykh ran i ustrojjstvo dlja ego osushhestvlenija // Vestnik MGTU im. N.EH. Baumana. Ser. Priborostroenie. 2015. № 6. S. 103−113.
  10. Sabelnikova T.M., Sabelnikov V.V., Gorjacheva V.N. Intensifikacija khimicheskogo vozdejjstvija nizkochastotnogo ultrazvuka na biologicheskie obekty // Biomedicinskaja radioehlektronika. 2016. № 7. S. 79−84
  11. KHorbenko I.D. Zvuk, ultrazvuk, infrazvuk. Izd. 2-e. M.: Znanie. 1996. 190 s.
  12. Pozdeev O.K. Medicinskaja mikrobiologija: Ucheb. posobie dlja vuzov. Izd. 4-e, ispr. / Pod red. V.I. Pokrovskogo. M.: GEHOTAR-Medija. 2007. 765 s.
  13. Goljamina I.P. Ultrazvuk: Malaja ehnciklopedija / Gl. red. I.P. Goljamina. M.: Sov. ehnciklopedija. 1989. 400 s.