O.V. Evseev - Post-graduate Student, MPTI Probationer Researcher, Kotel-nikov Institute of Radio Engineering, and Electronics Russian Academy of Sciences. E-mail: ev.mipt@gmail.com
S.A. Nikitov - Dr.Sc. (Phis.-Math.), Professor, Correspondent Member, Deputy Director, Kotel-nikov Institute of Radio Engineering, and Electronics Russian Academy of Sciences. E-mail: nikitov@cplire.ru
V.E. Antciperov - Ph.D. (Phis.-Math.), Senior Researcher, Kotel-nikov Institute of Radio Engineering, and Electronics Russian Academy of Sciences. E-mail: antciperov@cplire.ru

Results of the development of reconstruction algorithm of three-dimensional distributions of neurons, previously recognized by microscopic images of brain slices of animals, are presented. The adaptation of the EM-clustering algorithm to highlight groups of neurons in slices is considered. Authors propose a polynomial interlayer approximation of the parameters of two-dimensional neuronal distributions.