O.G. Tatarnikova – Post-graduate Student, Junior Research Scientist, Institute of Biophysics of a Cage  of RAS (Pushchino, Moscow Region)

E-mail: olga.psn@mail.ru

N.V. Bobkova – Ph.D. (Biol.), Head of Laboratory, Institute of Biophysics of a Cage  of RAS (Pushchino, Moscow Region)

E-mail: nbobkova@mail.ru

The cells producing toxic forms of Tau-protein and beta-amyloid were studied to develop an in vitro model of Alzheimer's disease (AD). By using of the immunocytochemical staining we have shown the ability of transfected 3T3-4R-Tau cells to produce Tauprotein characterized by pronounced toxicity that was detected during co-cultivation of 3T3-4R-Tau cells and primary culture of hippocampal cells from newborn rats. In addition, we have developed the cultivation technique for hippocampal cells from transgenic 5XFAD mice and demonstrated the ability of the cell culture to produce beta-amyloid.

1. Hussain I. APP transgenic mouse models and their use in drug discovery to evaluate amyloid- lowering therapeutics // CNS Neurol Disord Drug Targets. 2010. Aug. № 9(4). P. 395–402.
2. Kaushal A., Wani W.Y., Anand R., Gill K.D. Spontaneous and induced nontransgenic animal models of AD: modeling AD using combinatorial approach // Am. J. Alzheimers Dis Other Demen. 2013. Jun. № 28(4). P. 318–326.
3. Li Y. Establishment of experimental models for Alzheimer's disease research // Int. J. Neurosci. 2013 Dec. № 123(12). P. 823–831.
4. Lim S., Choi J.G., Moon M., Kim H.G., Lee W., Bak H.R., Sung H., Park C.H., Kim S.Y., Oh M.S. An Optimized Combination of Ginger and Peony Root Effectively Inhibits Amyloid-β Accumulation and Amyloid-β-Mediated Pathology in AβPP/PS1 Double-Transgenic Mice // J Alzheimers Dis. 2015. №50(1). P. 189–200. 
5. Bobkova N.V., Lyabin D.N., Medvinskaya N.I., Samokhin A.N., Nekrasov P.V., Nesterova I.V., Aleksandrova I.Y., Tatarnikova O.G., Bobylev A.G., Vikhlyantsev I.M., Kukharsky M.S., Ustyugov A.A., Polyakov D.N., Eliseeva I.A., Kretov D.A., Guryanov S.G., Ovchinnikov L.P. The Y-Box Binding Protein 1 Suppresses Alzheimer's Disease Progression in Two Animal Models // PLoS One. 2015. Sep. 22. №10(9). e0138867.
6. Tatarnikova O.G., Orlov M.A., Bobkova N.V. Beta-Amyloid and Tau-Protein: Structure, Interaction, and Prion-Like Properties // Biochemistry (Mosc). 2015. Dec. №80(13). P.1800–1819.