A.I. Vislobokov - Dr. Sc. (Biol.), Head of the Department of Neural Pharmacology, Senior Researcher Scientist, Institute of Pharmacology by A.V. Waldman named, Pavlov State Medical University of St.-Petersburg. E-mail: vislobokov@yandex.ru V.V. Marysheva - Dr. Sc. (Biol.), Senior Research Scientist, Military Medical Academy by S.M. Kirov named, (St.-Petersburg). Е-mail: vmarycheva@rambler.ru V.I. Orlov - Senior Research Scientist, Academy of Biology and Biotechnology, Southern Federal University (Rostov-on-Don). E-mail: orlov@rostel.ru P.D. Shabanov - Dr. Sc. (Med.), Head of the Department of Pharmacology, Research Institute of Experimental Medicine of the North-Western Branch of Russian Academy of Sciences (St.-Petersburg). E-mail: pdshabanov@mail.ru

In microelectrode studies and in the method of fixing the potential authors studied the effect on the neurons of pedal ganglia of a snail Planorbarius corneus amthizol succinate (SA) and amthizol disuccinate (DSA) in comparison with amthizol (A) at concentrations of 10, 100 and 1000 μM when the extracellular app. In the first part, they evaluated the dynamics of changes of resting potential (PP), impulsive activity (IA), the parameters of the action potential (AP) and total ion currents (in the first derivative AP - dV/dt). In the second - register changes the amplitudes of the incoming (sodium-calcium) in- and out- (slow potassium) ion currents and the nature of the changes in their kinetics of activation and inactivation (qualitatively). At concentrations of 10 and 100 μM they caused a similar dose-dependent and reversible hyperpolarization 1-4 mV, accompanied by a decrease in the frequency of AP without changing their duration and without reducing the total ion currents (dV/dt). At a concentration of 1000 μM A slightly (1-5 mV) depolarized neurons, reversible on 5-8 mV decreased the amplitude of AP. The amplitude of the sodium and calcium ionic currents is changed a little, at an action A potassium currents diminished on 7-15% from a norm. On membranotropic activity of a substance are arranged in a row: A > SA > DSA and draw conclusions about their ability to modulate the functional state of the cells.

 

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