B.Ya. Syropâtov - Dr.Sc. (Med.), Professor, Chair Department of Physiology of the Basics of Anatomy, Perm State Pharmaceutical Academy. E-mail: syropyatov@mail.ru N.V. Kolotova - Ph.D. (Chem.), Associate Professor, Department of Analytical Chemistry, Perm State Pharmaceutical Academy. E-mail: kolotova-nina@rambler.ru

Influence of succinic, maleic and phthalic acids derivatives on hemostasis have been already characterized in some literature. But there are no reports on influence of citraconic acid derivatives on hemostasis. Influence of 7 substituted amides and 3 citraconic acid acylhydrazides was analyzed with a help of «Minilab 701». The citrated (3.8 %) blood (9:1) was used. During the screening influence of these substances in concentration 1 mg/ml of blood on blood coagulation was analyzed. 8 animals were used in each set of tests. The solution of heparin in concentration 1 EU/ml of blood was used as an anticoagulative activity standard. The solution of etamsylate in concentration 1 mg/ml of blood was used as a haemostatic activity standard. The degree of anticoagulative and haemostasis activity of analyzed substances was figured out during measuring the time of citrated blood coagulation in check test-tube and experiment. The results were statistically worked up using STDEV. The analyzed substances have only haemostatic activity. Citraconic acid n-acetyl-phenyl amide showed the haemostatic activity that was 2 times greater than haemostatic activity of etamsylate. Carboxypentyl amide, antipyryl amide, triazolyl amide and citraconic acid iso-nicotinoyl hydrazide showed the haemostatic activity that was 1.4 times greater than haemostatic activity of ethanol. Citraconic acid 3-pyryl amide acts like etamsylate. Some mechanisms of structure influence of substances on haemostasis were figured out.

 

1. Patent № 2294199 (RF). 3-Metil-2-piridilamid maleinovojj kisloty, projavljajushhijj prjamoe antikoaguljantnoe dejjstvie / N.V. Kolotova, B.JA. Syropjatov, A.V. Dolzhenko, N.G.Ismajjlova.
2. Syropjatov B.JA., Kolotova N.V. Vlijanie na gemostaz proizvodnykh nekotorykh 1,4-dikarbonovykh kislot // XVI Rossijjskijj nacionalnyjj kongress «CHelovek i lekarstvo». M. 2009. 743 s.
3. Syropjatov B.JA., Kolotova N.V., Dolzhenko A.V. Vlijanie na gemostaz proizvonykh jantarnojj i maleinovojj kislot // Voprosy biologicheskojj, medicinskojj i farmacevticheskojj khimii. 2012. № 5. S. 31-36.
4. Syropjatov B.JA., Kolotova N.V. Vlijanie na gemostaz proizvodnykh ftalevykh kislot // Sb. «Innovacii v nauke»: materialy XVI Mezhdunar. zaoch. nauch.-prakt. konf. 2013. S. 139-146.
5. Dolzhenko A.V. Sintez, struktura i biologicheskaja aktivnost soedinenijj, poluchennykh na osnove reakcijj vzaimodejjstvija ciklicheskikh angidridov dikarbonovykh kislot s nukleofilnymi reagentami: Dis. ... kand. farm. nauk. 2003. Perm.
6. Sernov L.N., Gacura V.V. EHlementy ehksperimentalnojj farmakologii. M. 2000. S. 312-313.