A.A. Dutov - Dr.Sc. (Мed.), Senior Research, Laboratory of Experimental and Clinical Biochemistry and Immunology, Research Institute of Molecular Medicine, Chita State Medical Academy, Chita. E-mail: dutovaa@yandex.ru D.A. Nikitin - Head of the Laboratory of Chemical Analysis, Zabaikalsky State University, Chita. E-mail: nikitinnd@gmail.com P.P. Tereshkov - Ph. D. (Med.), Head of the Research Laboratory of Experimental and Clinical Biochemistry and Immunology, Research Institute of Molecular Medicine, Chita State Medical Academy, Chita. E-mail: tpp6915@mail.ru A.V. Martynova - Post-graduate Student, Department of Chemistry, Zabaikalsky State University, Chita A.V. Sverkunova - Post-graduate Student, Department of Chemistry, Zabaikalsky State University, Chita A.V. Yermolina - Physician Ophthalmologist, Regional Hospital № 2, Chita Yu.L. Lukyanova - Physician Ordinator, Department of Neurology, Chita State Medical Academy, Chita N.A. Shemyakina - Assistant, Department of Total Surgery, Chita State Medical Academy, Chita

A simple HPLC method for measuring of the total and free glutathione in red blood cells was validated. Reduction from disulfides was achieved with 1,4-dithioerythritol. Aminothiols derivatization was made with 5,5\'-dithio-bis-(2-nitrobenzoic acid). A derivative separated by means of is reversed-phase (С18) HPLC and UV detection at 330 nm. Simplicity and reproducibility of a method, allow its use in a clinical practice for an estimation of biochemical changes at various diseases.

 

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