Z.E. Maschenko - Ph.D. (Pharm.), Associate Professor, Department of Technology of Food Production and Perfumes and Cosmetic Products, Samara State Technical University. E-mail: mzinaida@yandex.ru
E.V. Maslova - Post-graduate Student, Samara State Technical University, Department of Technology of Food Production and Perfumes and Cosmetic Products, Samara State Technical University. E-mail: maslenok.08@mail.ru
P.G. Misina - Dr.Sc. (Biol.), Professor, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow). E-mail: mizina-pg@yandex.ru
Y.L. Gerasimov - Ph.D. (Biol.), Associate Professor, Head of the Department of Zoology, Genetics and General Ecology, Samara State Medical University. E-mail: zoo@front.ru
I.F. Shatalaev - Dr.Sc.(Biol.), Professor, Head of the Chemistry Department of Pharmaceutical Faculty, Samara State Medical University. E-mail: shatalaev@list.ru
P.P. Purygin - Dr.Sc.(Chem.), Professor, Head of the Department of Organic, Bioorganic and Medical Chemistry, Samara State University. E-mail: puryginpp2002@mail.ru

Currently, antibiotics productions occupy one of leading places among the products of the global pharmaceutical industry [1]. These compounds have low ability to biodegrade, and create conditions for selection of resistant microorganisms. The hazard class of environmental pollutants determination is the important question. One of the adopted experimental control methods for toxicity is the method of biotesting. As the test organisms used crayfish species Daphnia magna. Determination of the functional state of the active sludge  biological treatment plants is also possible with the biological tests. In particular, it can be used to modify the total dehydrogenase activity of the sludge [2]. Thus, research of evaluating the toxicity of medicinal substances contained in the wastewater towns, chemical-pharmaceutical and agro-industrial enterprises is important now. The aim of this work is to study the toxicity of benzylpenicillin sodium salt for live objects. The object of study was the sodium salt of benzylpenicillin. Crayfish species Dafnia magna aged up to 24 hours and the microorganisms of the activated sludge aeration tank treatment facilities were served the test organisms. The effect of benzylpenicillin sodium salt at Dafnia magna was conducted by the Stroganov-s method [2]. Determination of total dehydrogenase activity was performed according to the method based on the restoration indicator 2,3,5-triphenyltetrazolium chloride with subsequent photometric analysis of the obtained solutions at a wavelength of 490 nm [3]. All experiments were performed in 3 replications. In the experimentally determined threshold concentrations of benzylpenicillin sodium salt acting on the survival and reproduction of Dafnia magna. The threshold was 125 mg/l and 100 mg/l, respectively. The microorganisms were resistant to benzylpenicillin sodium salt. In the range of investigated concentrations of the toxic effect of the antibiotic is not installed. Information obtained by biotesting, is a tool for the adoption of environmentally sound technological solutions at the stage of sewage treatment in cities and industrial enterprises. This will save considerable resources to develop environmental measures for subsequent development of technologies for the production of chemical and pharmaceutical products.