350 rub
Journal №7 for 2014 г.
Article in number:
The Influence of low frequency physical factors on cell proliferation and cytokine gene expression
Keywords: cell cultures proliferation cytokines sound electromagnetic waves low and extremely high frequency
Authors:
O.A. Lopatina - Ph. D. (Med.), D.I. Ivanovsky Institute of Virology, Moscow. E-mail: cells@rambler.ru
M.V. Mezentseva - Dr. Sc. (Med.), D.I. Ivanovsky Institute of Virology, Moscow. E-mail: cells@rambler.ru
O.V. Baklanova - Ph.D. (Biol.), D.I. Ivanovsky Institute of Virology, Moscow. E-mail: cells@rambler.ru
R.Y. Podchernyaeva - Dr.Sc. (Med.), Professor
V.V. Egorov - Dr.Sc. (Chem.), Professor, The K.I. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology. E-mail: kaf-chimii@mgavm.ru
Abstract:
Growth-regulating activity of 8 cells lines (normal and tumor cells) after physical factors (acoustic waves, low frequency electromagnetic waves, extremely high frequency electromagnetic waves) exposure was investigated in this work. Also the impact of these physical factors on cytokine gene expression was learned. Acoustic waves with 50 Hz frequency led to proliferation index increase of all cells lines. Acoustic waves with 100 Hz frequency led to statistically significant proliferation index reduction. Cytokine gene silencing of INF-α and INF-γ was found after acoustic wave exposure in the range 50 to 10000 Hz. Acoustic wave exposure in the range 2000 to 10000 Hz led to cytokine gene expression increase of IL 1β, 2,4,6 and cytokine gene silencing of TNF-α. Low frequency electromagnetic waves (8 Hz, 72 Hz, 78,5 Hz) reduced growth-regulating activity neither normal nor tumor cells. Inhibitory activity of low frequency electromagnetic waves (in the presence of 3 frequencies) led to complete cells degeneration. Extremely high frequency electromagnetic waves exposure in the range 30 to 300 GHz activated neither normal nor tumor cells growth. These waves exposure during 5 minutes led to cytokine gene expression of TNF-α, IL2 and IL10. 15 minutes exposure time showed mRNA of IL12 synthesis. This cytokine play an important role in natural killer (NK) activation.
Pages: 22-27
References

  1. Mel'nichenko E.I., Osipova O.V., Podchernyaeva R.Ja. Ugnetenie rosta opuxolevy'x kletok pri e'kzogennom biorezonansnom vozdejstvii // Mezhdunarodnaya konferencziya «Teoreticheskie i klinicheskie aspekty' primeneniya biorezonansnoj i mul'tirezonansnoj terapii». 2002. Ch. II. S. 350-352.
  2. Podchernyaeva R.Ja., Mixajlova G.R., Isaeva E.I i dr. Primenenie KVCh oblucheniya v e'ksperimentax in vitro // Millimetrovy'e volny' v biologii i mediczine. 2004. № 1 (33). S. 12-17.
  3. Podchernyaeva R.Ja., Lopatina O.A., Mixajlova G.R. i dr. Vliyanie e'kzogennogo chastotnogo vozdejstviya na kletochny'e linii cheloveka // Kletochny'e texnologii v biologii i mediczine. 2008. № 3. S. 161-166.
  4. Lopatina O.A., Danly'baeva G.A., Baklanova O.V. i dr. E'kzogennoe biorezonansnoe vozdejstvie na kletochny'e kul'tury' // Materialy' VIII Mezhdunarodnogo kongressa «Zdorov'e i obrazovanie XXI vek. M-14-17 noyabrya 2007 g. S. 383-389.
  5. Egorov V.V., Lopatina O.A., Podchernyaeva R.Ja. Vliyanie zvuka akusticheskix chastot na rost kletochny'x kul'tur // Sb. Maly'e dozy' v biologii. M. 2009. S. 70-73.
  6. Egorov V.V. Nizkie chastoty' v biologii // problemnaya lekcziya FGOU MGAVM. B.M. 2007. S. 36-45.
  7. Gotovskij Ju.V., Kosareva L.V., Blinkov I.L., Samoxin A.V. E'kzogennaya biorezonansnaya terapiya fiksirovanny'mi chastotami. M.: IMEDIS. 2001.
  8. Metodicheskie rekomendaczii po rabote s kletochny'mi kul'turami (kul'tivirovanie pervichny'x i perevivaemy'x kletok) NII virusologii im. D.I. Ivanovskogo RAMN. M. 2009. S. 17.
  9. Lin Y., Zhang M., Barnes P.F. Chemokine production by a human alveolar epithelial cell line in response to Mycobacterium tuberculosis // Infection and Immunity. 1998. V. 66. № 3. P. 1121-1126.
  10. Gelder C.M., Thomas P.S., Yates D.H., Adcock I.M., Morrison J.F.J. et al. Cytokine expression in normal, atopic and asthmatic subject using the combination of sputum induction and the polymerase chain reaction. Thorax. 1995. V. 50. P. 1033-1037.
  11. Chomczynski N. Sacchi. Single-step method of RNA isolation by acid guanidinum thyocyoanate-phenol-chloroform extraction. Anal. Biochem. 1987. V. 162. P. 156-159.
  12. Devyatkov N.D., Arzumanov Ju.L., Beczkij O.V., Lebedeva N.N. Primenenie nizkointensivny'x e'lektromagnitny'x millimetrovy'x voln v mediczine // Zh. Millimetrovy'e volny' v mediczine i biologii. M. 1995. S. 6-8.
  13. Gotovskij Ju.V., Perov Ju.F. Osobennosti biologicheskogo dejstviya fizicheskix i ximicheskix faktorov maly'x i sverxmaly'x intensivnostej i doz. «Imedis». 2003. S. 78-90.