350 rub
Journal №4 for 2014 г.
Article in number:
Spontaneous oligonucleotide invasion into (CA/TG)n-repeats as the base for their function as homologous recombination «hot spots»
Keywords: (CA/TG)n-repeats homologous recombination oligonucleotide invasion
Authors:
N.Yu. Karprchenko - Assistant Researcher, Department of Chemical Carcinogenesis, Blokhin Cancer Research Center RAMS, Moscow. E-mail: nojadg@mail.ru
V.K. Gasanova - Senior Researcher, Department of Chemical carcinogenesis, Blokhin Cancer Research Center RAMS, Moscow. E-mail: victoriag@inbox.ru
D.S. Naberezhnov - Post-graduate Student, Department of Chemical Carcinogenesis, Blokhin Cancer Research Center RAMS. E-mail: n41d23s05@gmail.com
N.G. Dolinnaya - Dr. Sc. (Chem.), Leading Researcher, Department of Chemistry, Lomonosov Moscow State University. E-mail: dolinnaya@hotmail.com
M.G. Yakubovskaya - Dr.Sc. (Med.), Head of Department of Chemical Carcinogenesis, Blokhin Cancer Research Center RAMS, Moscow. E-mail: mgyakubovskaya@mail.ru
Abstract:
The main goal of our investigation is the study of molecular mechanism of homologous recombination, the main pathway for double strand breaks repair. We have demonstrated spontaneous invasion of d(GT)10 - oligonucleotides into (CA/TG)n repeats of genomic pro- and eukaryotic DNA that confirms unique structural properties of this type microsatellites and explains their function as recombination «hot spots». A number of other repetitive sequence oligonucleotides do not invade into duplex DNA. Only a small portion of duplex DNA interacts with oligonucleotide d(GT)10 or d(CA)10 and this interaction does not restore after DNA-oligonucleotide complex removing.
Pages: 30-35
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