T.S. Borovik - Post-graduate Student, National Research Tomsk State University. E-mail: Tomochka_07@mail.ru
L.N. Pribytkova - Dr.Sc. (Chem.), Professor, Siberian State Medical University (Tomsk). E-mail: PLN56@mail.ru

Gentianaceae family (Gentianaceae) has about 900 species belonging to 80 genera. In Siberia there are 10 genera Gentiana, including 41 species. Siberian species - is annual and perennial herbaceous plants that live in the highland and lowland meadows, steppes and tundra in less flattering communities [1]. Analysis of published data showed that the healing properties of gentian known since ancient times, but many of its species are not well understood. Our attention was drawn to the plant genus Gentiana [2]. Materials and methods. The objects of study were elevated part G. macrophylla Pall (1), G. cruciata L. (2), G. septemfida Pall (3). Samples were collected in August 2013 at the experimental site SibBS TSU. Raw dried to air-dry state. For obtaining extracts from test samples used 70% ethanol (the ratio of raw material and the extractant (1:10). For the detection of biologically active substances conducted qualitative reactions. Investigation of the obtained extracts is carried out by a two-dimensional paper chromatography (HD) and thin layer chromatography (TLC). Using chromatography paper - FN-11 (GDR), the plate Silufol UV-254 (Czech Republic). Two-dimensional chromatography performed in solvent systems: I - n-butanol: acetic acid: water (4: 1: 5) / 2% acetic acid; II - n-butanol: acetic acid: water (4: 1: 2) / 15% acetic acid. For thin layer chromatography, using a sorbent system III - chloroform: acetone (9 : 1). To display the chromatogram on the availability of different classes of biologically active compounds using specific reagents UV light. Quantitative analysis of phenolic acids, flavonoids, was carried out by spectrophotometry. The assay was performed using standard reference samples. The concentration of flavonoids, phenolic acids were determined based on the reference values of the specific absorption coefficient of luteolin and chlorogenic acid. For microscopic analysis using fresh material, the study sample was subjected leaves 1-3. Results and discussion. First conducted a comparative study of the anatomical G. macrophylla Pall, G. cruciata L., G. septemfida Pall., found that the cells of the upper and lower epidermis lamina samples 1-3 with strongly wobbling. Stomata are oval, surrounded by 4-5 okoloustichnymi cells (anomotsitny type), revealed differences in size of stomata, on the location of stomata on the leaf blade and the edges of the leaf blades. During the experiment, the method of qualitative reactions and BH in the system I, II and III in the TLC revealed that in G. macrophylla Pall., G. septemfida Pall., G. cruciata L. contains flavonoids, tannins, coumarins, phenolic acids, alkaloids. In all the studied samples are identified: apigenin, luteolin, caffeic and chlorogenic acid; p-coumaric and gallic acid - G. cruciata L., G. septemfida Pall; izohlorogenovaya acid - G. macrophylla Pall .; mangiferin. - G. cruciata L., G. septemfida Pall. Spectral analysis showed that the concentration of flavonoids and phenolic acids in the study of gentian is: G. septemfida Pall. 1.77% to 5.3%; G. cruciata L. 2,54%, 8,2%; G. macrophylla Pall. 2.08%, 1.1%, respectively.