350 rub
Journal №7 for 2013 г.
Article in number:
The influence of uv-light on the level of exspression of CD25 and on the production of interleukin-2 of lymphocytes of donor blood
Keywords: lymphocytes UV-light IL-2 CD25
Authors:
V.G. Artyukhov, O.V. Zemchenkova, O.V. Basharina, S.V. Ryazantsev
Abstract:
T-lymphocytes is the most numerous and heterogeneous by functional properties population of immune cells. Starting the proliferative response of T- lymphocytes is a multistage process in which the key role plays the expression of a T-cell growth factor − interleukin-2 and its receptor. The presence of cells carrying the marker CD25 (cells CD25+) in culture of human lymphocytes, indicates that the surface membrane of cells exhibited α-subunit of the IL-2 receptor. We have shown that in the course of daily incubation of cells the number of CD25+-cells increases. At the same time there is increase in the level of expression of CD25-receptors of photomodified lymphocytes in comparison to the control (non-irradiated cells). The addition of protein synthesis inhibitor - cycloheximide to the investigated system did not significantly change the level of CD25 expression in comparison to the values characteristic of the non-irradiated cells. Thus, the registered increase of the expression of the investigated marker was associated with de novo synthesis of the receptor. UV-radiation causes an increase of ROS. Probably, ROS activate transcription factors and initiate protein synthesis de novo. Moreover, it is known that in the cell UV-light may increase the concentration of calcium ions, and calcium signal plays a key role in the activation of the transcription factor NFAT (nuclear factor of activated cells) and in the expression of a growth factor IL-2 and its receptor (IL-2R). Analysis of IL-2 concentration demonstrated that the production of this cytokine by photomodified lymphocytes increased by 2,8 and 3,6 times correspondingly to the radiation doses (151 and 755 J/m2) in comparison to the control. Reception by activated cells with IL-2 generates signals needed to start the proliferation and differentiation of activated cells, as well as to block apoptosis. Thus, UV-light in the range of doses used, induces the expression of the growth factor IL-2 and its receptor. IL-2 produced by photomodified lymphocytes is the main autocrine factor of T-lymphocytes, which protects activated T-cells from apoptosis. Apparently, the increased number of CD25+-molecules observed during incubation of the cells designed to protect T-cells from the development of ROS-mediated apoptosis.
Pages: 48-52
References

  1. Karandashov V.I., Petukhxov E.B., Zrodnikov V.S. Fototerapiya. M.: Mediczina. 2001. 392 s.
  2. Berridge M.J. Lymphocyte activation in health and disease // Crit. Rev. Immunol. 1997. V. 17. P. 155-178.
  3. Leonard W.J., Lin J.X. Cytokine receptor signaling pathway // J. Allergy Clin. Immunol. 2000. V. 105. P. 877-888.
  4. Eicher D.M., Damjanovich S., Waldmann T.A Oligomerization of IL-2Ralpha // Cytokine. 2002. V. 17. P. 82-90.
  5. Benczik M., Gaffen S.L. The interleukin (IL)-2 family cytokines: survival and proliferation signaling pathways in T lymphocytes // Immunol. Invest. 2004. V. 33. P. 109-142.
  6. Kim H.P., Leonard W.J. The basis of TCR-mediated regulation of the IL-2 receptor alpha chain gene: role of widely separated regulatory elements // EMBO J. 2002. V. 21. P. 3051-3059.
  7. Kim H.P., Imbert J., Leonard. W.J. Both integrated and differential regulation of components of IL-2/IL-2 receptor system // Cytokine Growth Factor Rev. 2006. V. 17. P. 350-366.
  8. Artyukxov V.G., Zemchenkova O.V., Basharina O.V., Kim Ja.V. Osobennosti metabolizma UF-obluchenny'x limfoczitov // Radiaczionnaya biologiya. Radioe'kologiya. 2011. T. 51. № 2. S. 252-257.
  9. Imbert V., Renauld P.R. Duration of STAT5 activation influences the response of interleukin-2 receptor alpha gene to different cytokines // Eur. Cytokine Netw. 1999. V. 10. P. 71-78.
  10. Matikainen S., Sareneva T., Ronni T., Lehtonen A., Koskinen P.J., Julkunen I. Interferon-alpha activates multiple STAT proteins and upregulates proliferation-associated IL-2Ralpha, c-myc, and pim-1 genes in human T cells // Blood. 1999. V. 93. P. 1980-1991.
  11. John S., Vinkemeier U., Soldani E., Darnell J.C., Leonard W.J. The significance of tetramerization in promoter recruitment by Stat5 // Mol. Cell Biol. 1999. V. 19. P. 1910-1918.
  12. Ahmed N.N., Grimes H.L., Bellacosa A., Chan T.O., Tsichlis P.N. Transduction of interleukin-2 antiapoptotic and proliferative signals via Akt protein kinase // Proc. Nat. Acad. Sci. USA. 1997. V. 94. P. 3627-3632.
  13. Yu C.R., Ortaldo J.R., Curiel R.E., Young H.A., Anderson S.K., Gosselin P. Role of a STAT binding site in the regulation of the human perforin promoter // J. Immunol. 1999. V. 162. P. 2785-2790.
  14. Kawahara A., Minami Y., Miyazaki T., Ihle J.N., Taniguchi T. Critical role of the interleukin 2 (IL-2) receptor gamma-chain-associated Jak3 in the IL-2-induced c-fos and c-myc, but not bcl-2, gene induction // Proc. Nat. Acad. Sci. USA. 1995. V. 92. P. 8724-8728.
  15. Turka L.A., Fletcher M.C., Graighead N. Defective signal transduction by the CD2 molecule in immature T-cell receptor/CD3- thymocytes // Proc. Nat. Acad. Sci. USA. 1992. V. 89. P. 8706-8718.
  16. Galaktionov K., Chen X., Beach D.Cdc25 cell-cycle phosphatase as a target of c-myc // Nature. 1996. V. 382. P. 511-517.
  17. Castro A., Sengupta T.K., Ruiz D.C., Yang E., Ivashkiv L.B. IL-4 selectively inhibits IL-2-triggered Stat5 activation, but not proliferation, in human T cells // J. Immunol. 1999. V. 162. P. 1261-1269.
  18. Bradshaw J.M. The Src, Syk, and Tec family kinases: distinct types of molecular switches // Cell Signal. 2010. V. 22. P. 1175-1184.
  19. Smith-Garvin J.E., Koretzky G.A., Jordan M.S. T cell activation // Annu. Rev. Immunol. 2009. V. 27. P. 591-619.
  20. Rane S.G., Reddy E.P. JAKs, STATs and Src kinases in hematopoiesis // Oncogene. 2002. V. 21. P. 3334-3358.
  21. Ellery G.M., Nicholls P.J. Possible mechanism for the alpha subunit of the interleukin-2 receptor (CD25) to influence interleukin-2 receptor signal transduction // Immunol. Cell Biol. 2002. V. 80. P. 351-357.
  22. Artyukhov V.G., Zemchenkova O.V., Basharina O.V., Ryazanczev S.V. Vliyanie UF-sveta na reczeptorny'j profil' limfoczitov donorskoj krovi // Radiaczionnaya biologiya. Ser. Radioe'kologiya. 2012. T. 52. № 5. S. 534-541.
  23. Ahn H.J., Tomura M., Yu W.G., Iwasaki M., Park W.R., Hamaoka T., Fujiwara H. Requirement for distinct Janus kinases and STAT proteins in T cell proliferation versus IFN-gamma production following IL-12 stimulation // J. Immunol. 1998. V. 161. P. 5893-5900.